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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Acta+Crystallogr+D+Struct+Biol
2016 ; 72
(Pt 3
): 359-74
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gab.com Text
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Can I solve my structure by SAD phasing? Planning an experiment, scaling data and
evaluating the useful anomalous correlation and anomalous signal
#MMPMID26960123
Terwilliger TC
; Bunkóczi G
; Hung LW
; Zwart PH
; Smith JL
; Akey DL
; Adams PD
Acta Crystallogr D Struct Biol
2016[Mar]; 72
(Pt 3
): 359-74
PMID26960123
show ga
A key challenge in the SAD phasing method is solving a structure when the
anomalous signal-to-noise ratio is low. Here, algorithms and tools for evaluating
and optimizing the useful anomalous correlation and the anomalous signal in a SAD
experiment are described. A simple theoretical framework [Terwilliger et al.
(2016), Acta Cryst. D72, 346-358] is used to develop methods for planning a SAD
experiment, scaling SAD data sets and estimating the useful anomalous correlation
and anomalous signal in a SAD data set. The phenix.plan_sad_experiment tool uses
a database of solved and unsolved SAD data sets and the expected characteristics
of a SAD data set to estimate the probability that the anomalous substructure
will be found in the SAD experiment and the expected map quality that would be
obtained if the substructure were found. The phenix.scale_and_merge tool scales
unmerged SAD data from one or more crystals using local scaling and optimizes the
anomalous signal by identifying the systematic differences among data sets, and
the phenix.anomalous_signal tool estimates the useful anomalous correlation and
anomalous signal after collecting SAD data and estimates the probability that the
data set can be solved and the likely figure of merit of phasing.