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10.1101/pdb.prot066670 http://scihub22266oqcxt.onion/10.1101/pdb.prot066670 C4784430!4784430!22046049     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cold+Spring+Harb+Protoc 2011 ; 2011 (11): 1370-1 Nephropedia Template TP {{tp|p=22046049|t=2011. Attachment of Anti-GFP Antibodies to Microspheres for Optical Trapping Experiments |pdf=|usr=}}{{22046049}} gab.com Text Attachment of Anti-GFP Antibodies to Microspheres for Optical Trapping Experiments JO: Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22046049 #FOAvip In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes a method for attaching anti-GFP (green fluorescent protein) antibodies to microspheres. GFP-motor fusion proteins can then be adsorbed to the microspheres for use in single-molecule motility studies and optical trapping experiments. Twit Text FOAVip Attachment of Anti-GFP Antibodies to Microspheres for Optical Trapping Experiments ????Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22046049 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22046049 #FOAvip English Wikipedia <ref>{{Cite pmid|22046049}}</ref> Attachment of Anti-GFP Antibodies to Microspheres for Optical Trapping Experiments #MMPMID22046049 Spudich JA; Rice SE; Rock RS; Purcell TJ; Warrick HM Cold Spring Harb Protoc 2011[Nov]; 2011 (11): 1370-1 PMID22046049show ga In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes a method for attaching anti-GFP (green fluorescent protein) antibodies to microspheres. GFP-motor fusion proteins can then be adsorbed to the microspheres for use in single-molecule motility studies and optical trapping experiments. äAttachment of Anti-GFP Antibodies to Microspheres for Optical Trapping(epmc).pdf DeepDyve Pubget Overpricing