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10.1186/s12944-016-0216-5 http://scihub22266oqcxt.onion/10.1186/s12944-016-0216-5 C4784424!4784424!26956882     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lipids+Health+Dis 2016 ; 15 (ä): ä Nephropedia Template TP {{tp|p=26956882|t=2016. MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells |pdf=|usr=}}{{26956882}} gab.com Text MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells JO: Lipids Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=26956882 #FOAvip Background: Recent discoveries of the atherosclerosis-related miRNAs shed new light on the treatment of cardiovascular diseases. Of note, miR-106b?~?25 cluster and miR-17?~?92 cluster are paralogs. Up till now, plenty of researches have shown the role of miR-17?~?92 cluster in tumor and atherosclerosis, but miR-106b?~?25 cluster has stayed mysterious in atherosclerosis field. This study was designed to investigate how miR-106b functions in the atherosclerosis-related angiogenesis and to explore the functioning processes of miR-106b, so as to seek out a new target for the treatment of atherosclerosis. Methods: Up and down regulation of miR-106b expression was achieved through transfection in HUVECs so as to investigate the function of miR-106b. Next we predicted the target genes of miR-106b and detected them using qRT-PCR and Western blot technique. At last, luciferase assay was conducted to verify the direct target gene of miR-106b. Data are expressed as mean?±?SEM. Two treatment groups were compared by Mann?Whitney U test or student?s t-test. Results were considered statistically significant when P?<?0.05. Results: The results showed miR-106b up-regulation groups formed less tubes than control groups while the down-regulation groups showed the opposite. Meanwhile, no obvious effect on apoptosis was observed in endothelial cells. Next we predicted the target genes of miR-106b and finally settled down to MAPK14 (Mitogen-Activated Protein Kinase), STAT3 (Signal Transducers and Activators of Transcription 3), JAK1(Janus Kinase 1) and VEGFA(Vascular Endothelial Growth Factor A) as candidate target genes. Our results revealed over-expressed miR-106b represses STAT3 expression, while miR-106b inhibition resulted in STAT3 up-regulation. Ultimately, luciferase assay confirmed STAT3 mRNA is the direct target of miR-106b. Conclusions: Our research demonstrated that miR-106b modulate angiogenesis in endothelial cells through affecting expression of STAT3, which occurs by direct target action. Therefore, we affirmed that miR-106b exerts an anti-angiogenic effect in endothelial cells via STAT3-involved signaling pathway, via directly targeting STAT3. Twit Text FOAVip MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells ????Lipids Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=26956882 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26956882 #FOAvip English Wikipedia <ref>{{Cite pmid|26956882}}</ref> MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells #MMPMID26956882 Maimaiti A; Maimaiti A; Yang Y; Ma Y Lipids Health Dis 2016[]; 15 (ä): ä PMID26956882show ga Background: Recent discoveries of the atherosclerosis-related miRNAs shed new light on the treatment of cardiovascular diseases. Of note, miR-106b?~?25 cluster and miR-17?~?92 cluster are paralogs. Up till now, plenty of researches have shown the role of miR-17?~?92 cluster in tumor and atherosclerosis, but miR-106b?~?25 cluster has stayed mysterious in atherosclerosis field. This study was designed to investigate how miR-106b functions in the atherosclerosis-related angiogenesis and to explore the functioning processes of miR-106b, so as to seek out a new target for the treatment of atherosclerosis. Methods: Up and down regulation of miR-106b expression was achieved through transfection in HUVECs so as to investigate the function of miR-106b. Next we predicted the target genes of miR-106b and detected them using qRT-PCR and Western blot technique. At last, luciferase assay was conducted to verify the direct target gene of miR-106b. Data are expressed as mean?±?SEM. Two treatment groups were compared by Mann?Whitney U test or student?s t-test. Results were considered statistically significant when P? äMiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expr(epmc).pdf DeepDyve Pubget Overpricing