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10.1111/cmi.12130 http://scihub22266oqcxt.onion/10.1111/cmi.12130 C4784422!4784422!23470014     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Microbiol 2013 ; 15 (8): 1427-37 Nephropedia Template TP {{tp|p=23470014|t=2013. Staphylococcal alpha-Phenol Soluble Modulins contribute to neutrophil lysis after phagocytosis |pdf=|usr=}}{{23470014}} gab.com Text Staphylococcal alpha-Phenol Soluble Modulins contribute to neutrophil lysis after phagocytosis JO: Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=23470014 #FOAvip Staphylococcus aureus community-acquired (CA) MRSA strains are highly virulent and can cause infections in otherwise healthy individuals. The most important mechanism of the host for clearing S. aureus is phagocytosis by neutrophils and subsequent killing of the pathogen. Especially CA-MRSA strains are very efficient in circumventing this neutrophil killing. Interestingly, only a relative small number of virulence factors have been associated with CA-MRSA, one of which are the phenol soluble modulins (PSMs). We have recently shown that the PSMs are functionally inhibited by serum lipoproteins, indicating that PSMs may exert their cytolytic function primarily in the intracellular environment. To further investigate the intracellular role of the PSMs we measured the effect of the ?-type and ?-type PSMs on neutrophil killing after phagocytosis. Using fluorescently labeled S. aureus, we measured bacterial survival after phagocytosis in a plate reader, which was employed next to flow cytometry and time-lapse microscopy. Phagocytosis of the CA-MRSA strain MW2 by human neutrophils resulted in rapid host cell death. Using mutant strains of MW2, we demonstrated that in the presence of serum, the intracellular expression of only the psm? operon is both necessary and sufficient for both increased neutrophil cell death and increased survival of S. aureus. Our results identify PSM? peptides as prominent contributors to killing of neutrophils after phagocytosis, a finding with major implications for our understanding of S. aureus pathogenesis and strategies for S. aureus vaccine development. Twit Text FOAVip Staphylococcal alpha-Phenol Soluble Modulins contribute to neutrophil lysis after phagocytosis ????Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=23470014 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23470014 #FOAvip English Wikipedia <ref>{{Cite pmid|23470014}}</ref> Staphylococcal alpha-Phenol Soluble Modulins contribute to neutrophil lysis after phagocytosis #MMPMID23470014 Surewaard B; de Haas C; Vervoort F; Rigby K; DeLeo F; Otto M; van Strijp J; Nijland R Cell Microbiol 2013[Aug]; 15 (8): 1427-37 PMID23470014show ga Staphylococcus aureus community-acquired (CA) MRSA strains are highly virulent and can cause infections in otherwise healthy individuals. The most important mechanism of the host for clearing S. aureus is phagocytosis by neutrophils and subsequent killing of the pathogen. Especially CA-MRSA strains are very efficient in circumventing this neutrophil killing. Interestingly, only a relative small number of virulence factors have been associated with CA-MRSA, one of which are the phenol soluble modulins (PSMs). We have recently shown that the PSMs are functionally inhibited by serum lipoproteins, indicating that PSMs may exert their cytolytic function primarily in the intracellular environment. To further investigate the intracellular role of the PSMs we measured the effect of the ?-type and ?-type PSMs on neutrophil killing after phagocytosis. Using fluorescently labeled S. aureus, we measured bacterial survival after phagocytosis in a plate reader, which was employed next to flow cytometry and time-lapse microscopy. Phagocytosis of the CA-MRSA strain MW2 by human neutrophils resulted in rapid host cell death. Using mutant strains of MW2, we demonstrated that in the presence of serum, the intracellular expression of only the psm? operon is both necessary and sufficient for both increased neutrophil cell death and increased survival of S. aureus. Our results identify PSM? peptides as prominent contributors to killing of neutrophils after phagocytosis, a finding with major implications for our understanding of S. aureus pathogenesis and strategies for S. aureus vaccine development. äStaphylococcal alpha-Phenol Soluble Modulins contribute to neutrophil (epmc).pdf DeepDyve Pubget Overpricing