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10.1007/978-1-61779-400-1_20

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C4783134!4783134!22130889
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suck abstract from ncbi


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pmid22130889      Methods+Mol+Biol 2012 ; 802 (ä): 305-22
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  • ChIP-Seq Data Analysis: Identification of Protein?DNA Binding Sites with SISSRs Peak-Finder #MMPMID22130889
  • Narlikar L; Jothi R
  • Methods Mol Biol 2012[]; 802 (ä): 305-22 PMID22130889show ga
  • Protein?DNA interactions play key roles in determining gene-expression programs during cellular development and differentiation. Chromatin immunoprecipitation (ChIP) is the most widely used assay for probing such interactions. With recent advances in sequencing technology, ChIP-Seq, an approach that combines ChIP and next-generation parallel sequencing is fast becoming the method of choice for mapping protein?DNA interactions on a genome-wide scale. Here, we briefly review the ChIP-Seq approach for mapping protein?DNA interactions and describe the use of the SISSRs peak-finder, a software tool for precise identification of protein?DNA binding sites from sequencing data generated using ChIP-Seq.
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