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10.1101/pdb.prot066688 http://scihub22266oqcxt.onion/10.1101/pdb.prot066688 C4782787!4782787!22046050     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cold+Spring+Harb+Protoc 2011 ; 2011 (11): 1372-4 Nephropedia Template TP {{tp|p=22046050|t=2011. The Optical Trapping Dumbbell Assay for Nonprocessive Motors or Motors That Turn around Filaments |pdf=|usr=}}{{22046050}} gab.com Text The Optical Trapping Dumbbell Assay for Nonprocessive Motors or Motors That Turn around Filaments JO: Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22046050 #FOAvip In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes the preparation of biotin?actin filaments and coverslips coated with polystyrene beads. These are then used in optical trapping dumbbell assays to study interactions between motors and filaments. Twit Text FOAVip The Optical Trapping Dumbbell Assay for Nonprocessive Motors or Motors That Turn around Filaments ????Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22046050 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22046050 #FOAvip English Wikipedia <ref>{{Cite pmid|22046050}}</ref> The Optical Trapping Dumbbell Assay for Nonprocessive Motors or Motors That Turn around Filaments #MMPMID22046050 Spudich JA; Rice SE; Rock RS; Purcell TJ; Warrick HM Cold Spring Harb Protoc 2011[Nov]; 2011 (11): 1372-4 PMID22046050show ga In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes the preparation of biotin?actin filaments and coverslips coated with polystyrene beads. These are then used in optical trapping dumbbell assays to study interactions between motors and filaments. äThe Optical Trapping Dumbbell Assay for Nonprocessive Motors or Motors(epmc).pdf DeepDyve Pubget Overpricing