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10.1073/pnas.1517883113 http://scihub22266oqcxt.onion/10.1073/pnas.1517883113 C4780610!4780610!26864203     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2016 ; 113 (9): 2544-9 Nephropedia Template TP {{tp|p=26864203|t=2016. Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM |pdf=|usr=}}{{26864203}} gab.com Text Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26864203 #FOAvip The systematic discovery of new gene and drug combinations that modulate complex biological phenotypes and human diseases requires scalable and multiplexed screening technologies. We leverage the programmability of the CRISPR-Cas9 system for multiplexed targeting of specific genomic loci and the versatility of the combinatorial genetics en masse (CombiGEM) technology to rapidly assemble barcoded combinatorial genetic perturbation libraries that can be tracked with high-throughput sequencing. CombiGEM-CRISPR enables simple, massively parallel screening of barcoded combinatorial gene perturbations in human cells, and the translation of these hits into effective drug combinations. This approach is broadly applicable for performing pooled combinatorial genetic perturbations to map out how the orchestrated action of genes controls complex phenotypes and to translate these findings into novel drug combinations. Twit Text FOAVip Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26864203 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26864203 #FOAvip English Wikipedia <ref>{{Cite pmid|26864203}}</ref> Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM #MMPMID26864203 Wong ASL; Choi GCG; Cui CH; Pregernig G; Milani P; Adam M; Perli SD; Kazer SW; Gaillard A; Hermann M; Shalek AK; Fraenkel E; Lu TK Proc Natl Acad Sci U S A 2016[Mar]; 113 (9): 2544-9 PMID26864203show ga The systematic discovery of new gene and drug combinations that modulate complex biological phenotypes and human diseases requires scalable and multiplexed screening technologies. We leverage the programmability of the CRISPR-Cas9 system for multiplexed targeting of specific genomic loci and the versatility of the combinatorial genetics en masse (CombiGEM) technology to rapidly assemble barcoded combinatorial genetic perturbation libraries that can be tracked with high-throughput sequencing. CombiGEM-CRISPR enables simple, massively parallel screening of barcoded combinatorial gene perturbations in human cells, and the translation of these hits into effective drug combinations. This approach is broadly applicable for performing pooled combinatorial genetic perturbations to map out how the orchestrated action of genes controls complex phenotypes and to translate these findings into novel drug combinations. äMultiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM (epmc).pdf DeepDyve Pubget Overpricing