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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nephrourol+Mon
2016 ; 8
(1
): e34421
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Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular
Regeneration After Renal Ischemia/Reperfusion Injury in Rats
#MMPMID26981502
Migita H
; Yoshitake S
; Tange Y
; Choijookhuu N
; Hishikawa Y
Nephrourol Mon
2016[Jan]; 8
(1
): e34421
PMID26981502
show ga
BACKGROUND: Renal ischemia/reperfusion (I/R) injury remains a major cause of
acute kidney injury (AKI), in addition to I/R injury-induced tissue inflammation,
necrosis and apoptosis. Hyperbaric oxygen therapy (HBO) is defined as a treatment
in which a patient is intermittently exposed to 100% oxygen pressurized to a
pressure above sea level (> 2.0 atmospheres absolute (ATA), 1.0 ATA = 760 mmHg).
It has been used in a number of medical conditions with a proven efficacy in a
limited number of disorders. However, the effects of HBO therapy on apoptosis and
proliferative activity after I/R injury have not been fully understood.
OBJECTIVES: We studied the possible beneficial effects of HBO therapy on
apoptosis and tubular cell regeneration after renal I/R injury in rats. MATERIALS
AND METHODS: Sprague-Dawley (SD) rats were randomized into three groups: Sham
(Sham-operated rats); I/R (animals submitted to I/R); and I/R + HBO (I/R rats
exposed to HBO). Tubular cell apoptosis was confirmed by DNA laddering and the
terminal deoxynucleotidyl transferase-mediated uridine triphosphate nick end
labeling (TUNEL) assay. Cellular proliferation activity was determined using the
anti-Ki-67 antibody. RESULTS: A significant decrease in apoptotic cells and
increase in proliferative reaction were observed in the I/R + HBO group compared
to the I/R group. CONCLUSIONS: We demonstrated that HBO suppressed apoptosis,
which caused inflammation after renal I/R, and promoted tubular cell
regeneration. HBO has protective effects against AKI caused by renal I/R through
the inhibition of apoptosis.