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10.1093/infdis/jiv565

http://scihub22266oqcxt.onion/10.1093/infdis/jiv565
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C4779302!4779302!26715676
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suck abstract from ncbi


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pmid26715676      J+Infect+Dis 2016 ; 213 (7): 1124-33
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  • Development of a Cost-effective Ovine Polyclonal Antibody-Based Product, EBOTAb, to Treat Ebola Virus Infection #MMPMID26715676
  • Dowall SD; Callan J; Zeltina A; Al-Abdulla I; Strecker T; Fehling SK; Krähling V; Bosworth A; Rayner E; Taylor I; Charlton S; Landon J; Cameron I; Hewson R; Nasidi A; Bowden TA; Carroll MW
  • J Infect Dis 2016[Apr]; 213 (7): 1124-33 PMID26715676show ga
  • The highly glycosylated glycoprotein spike of Ebola virus (EBOV-GP1,2) is the primary target of the humoral host response. Recombinant EBOV-GP ectodomain (EBOV-GP1,2ecto) expressed in mammalian cells was used to immunize sheep and elicited a robust immune response and produced high titers of high avidity polyclonal antibodies. Investigation of the neutralizing activity of the ovine antisera in vitro revealed that it neutralized EBOV. A pool of intact ovine immunoglobulin G, herein termed EBOTAb, was prepared from the antisera and used for an in vivo guinea pig study. When EBOTAb was delivered 6 hours after challenge, all animals survived without experiencing fever or other clinical manifestations. In a second series of guinea pig studies, the administration of EBOTAb dosing was delayed for 48 or 72 hours after challenge, resulting in 100% and 75% survival, respectively. These studies illustrate the usefulness of EBOTAb in protecting against EBOV-induced disease.
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