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10.3748/wjg.v22.i9.2779 http://scihub22266oqcxt.onion/10.3748/wjg.v22.i9.2779 C4778000!4778000!26973416     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       World+J+Gastroenterol 2016 ; 22 (9): 2779-88 Nephropedia Template TP {{tp|p=26973416|t=2016. New role and molecular mechanism of Gadd45a in hepatic ?brosis |pdf=|usr=}}{{26973416}} gab.com Text New role and molecular mechanism of Gadd45a in hepatic brosis JO: World J Gastroenterol http://www.kidney.de/pget.php?&tw=1&pmid=26973416 #FOAvip AIM: To investigate the role of Gadd45a in hepatic ?brosis and the transforming growth factor (TGF)-?/Smad signaling pathway.METHODS: Wild-type male BALB/c mice were treated with CCl4 to induce a model of chronic liver injury. Hepatic stellate cells (HSCs) were isolated from the liver of BALB/c mice and were treated with small interfering RNAs (siRNAs) targeting Gadd45a or the pcDNA3.1-Gadd45a recombinant plasmid. Cellular ?-smooth muscle actin (?-SMA), ?-actin, type?I?collagen, phospho-Smad2, phospho-Smad3, Smad2, Smad3, and Smad4 were detected by Western blots. The mRNA levels of ?-SMA, ?-actin, and type?I?collagen were determined by quantitative real-time (qRT)-PCR analyses. Reactive oxygen species production was monitored by ?ow cytometry using 2,7-dichlorodihydro?uorescein diacetate. Gadd45a, Gadd45b, anti-Gadd45g, type?I?collagen, and SMA local expression in liver tissue were measured by histologic and immunohistochemical analyses.RESULTS: Significant downregulation of Gadd45a, but not Gadd45b or Gadd45g, accompanied by activation of the TGF-?/Smad signaling pathways was detected in fibrotic liver tissues of mice and isolated HSCs with chronic liver injury induced by CCl4 treatment. Overexpression of Gadd45a reduced the expression of extracellular matrix proteins and ?-SMA in HSCs, whereas transient knockdown of Gadd45a with siRNA reversed this process. Gadd45a inhibited the activity of a plasminogen activator inhibitor-1 promoter construct and (CAGA)9 MLP-Luc, an artificial Smad3/4-specific reporter, as well as reduced the phosphorylation and nuclear translocation of Smad3. Gadd45a showed protective effects by scavenging reactive oxygen species and upregulating antioxidant enzymes.CONCLUSION: Gadd45a may counteract hepatic ?brosis by regulating the activation of HSCs via the inhibition of TGF-?/Smad signaling. Twit Text FOAVip New role and molecular mechanism of Gadd45a in hepatic brosis ????World J Gastroenterol http://www.kidney.de/pget.php?&tw=1&pmid=26973416 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26973416 #FOAvip English Wikipedia <ref>{{Cite pmid|26973416}}</ref> New role and molecular mechanism of Gadd45a in hepatic ?brosis #MMPMID26973416 Hong L; Sun QF; Xu TY; Wu YH; Zhang H; Fu RQ; Cai FJ; Zhou QQ; Zhou K; Du QW; Zhang D; Xu S; Ding JG World J Gastroenterol 2016[Mar]; 22 (9): 2779-88 PMID26973416show ga AIM: To investigate the role of Gadd45a in hepatic ?brosis and the transforming growth factor (TGF)-?/Smad signaling pathway.METHODS: Wild-type male BALB/c mice were treated with CCl4 to induce a model of chronic liver injury. Hepatic stellate cells (HSCs) were isolated from the liver of BALB/c mice and were treated with small interfering RNAs (siRNAs) targeting Gadd45a or the pcDNA3.1-Gadd45a recombinant plasmid. Cellular ?-smooth muscle actin (?-SMA), ?-actin, type?I?collagen, phospho-Smad2, phospho-Smad3, Smad2, Smad3, and Smad4 were detected by Western blots. The mRNA levels of ?-SMA, ?-actin, and type?I?collagen were determined by quantitative real-time (qRT)-PCR analyses. Reactive oxygen species production was monitored by ?ow cytometry using 2,7-dichlorodihydro?uorescein diacetate. Gadd45a, Gadd45b, anti-Gadd45g, type?I?collagen, and SMA local expression in liver tissue were measured by histologic and immunohistochemical analyses.RESULTS: Significant downregulation of Gadd45a, but not Gadd45b or Gadd45g, accompanied by activation of the TGF-?/Smad signaling pathways was detected in fibrotic liver tissues of mice and isolated HSCs with chronic liver injury induced by CCl4 treatment. Overexpression of Gadd45a reduced the expression of extracellular matrix proteins and ?-SMA in HSCs, whereas transient knockdown of Gadd45a with siRNA reversed this process. Gadd45a inhibited the activity of a plasminogen activator inhibitor-1 promoter construct and (CAGA)9 MLP-Luc, an artificial Smad3/4-specific reporter, as well as reduced the phosphorylation and nuclear translocation of Smad3. Gadd45a showed protective effects by scavenging reactive oxygen species and upregulating antioxidant enzymes.CONCLUSION: Gadd45a may counteract hepatic ?brosis by regulating the activation of HSCs via the inhibition of TGF-?/Smad signaling. äNew role and molecular mechanism of Gadd45a in hepatic ?brosis (epmc).pdf DeepDyve Pubget Overpricing