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Effects of Different Doses of Levetiracetam on Aquaporin 4 Expression in Rats
with Brain Edema Following Fluid Percussion Injury
#MMPMID26927633
Jin H
; Li W
; Dong C
; Ma L
; Wu J
; Zhao W
Med Sci Monit
2016[Feb]; 22
(?): 678-86
PMID26927633
show ga
BACKGROUND This study was designed to investigate the effects of different doses
of levetiracetam on aquaporin 4 (AQP4) expression in rats after fluid percussion
injury. MATERIAL AND METHODS Sprague-Dawley rats were randomly divided into 4
groups: sham operation group, traumatic brain injury group, low-dose
levetiracetam group, and high-dose levetiracetam group. Brain edema models were
established by fluid percussion injury, and intervened by the administration of
levetiracetam. Samples from the 4 groups were collected at 2, 6, 12, and 24 h,
and at 3 and 7 days after injury. Histological observation was performed using
hematoxylin-eosin staining and immunohistochemical staining. AQP4 and AQP4 mRNA
expression was detected using Western blot assay and RT-PCR. Brain water content
was measured by the dry-wet method. RESULTS Compared with the traumatic brain
injury group, brain water content, AQP4 expression, and AQP4 mRNA expression were
lower in the levetiracetam groups at each time point and the differences were
statistically significant (P<0.05). The intervention effects of high-dose
levetiracetam were more apparent. CONCLUSIONS Levetiracetam can lessen brain
edema from fluid percussion injury by down-regulating AQP4 and AQP4 mRNA
expression. There is a dose-effect relationship in the preventive effect of
levetiracetam within a certain extent.
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