Pathological Analysis of Cell Differentiation in Cholesterol Granulomas
Experimentally Induced in Mice
#MMPMID26941582
Sakai K
; Nakano K
; Matsuda S
; Tsujigiwa H
; Ochiai T
; Shoumura M
; Osuga N
; Hasegawa H
; Kawakami T
Int J Med Sci
2016[]; 13
(3
): 220-4
PMID26941582
show ga
In this study, cholesterin was implanted in the subcutaneous tissue in mice to
induce the formation of cholesterol granuloma. Histological examination was
carried out to determine the type and source of cells. The tissue surrounding the
embedded cholesterin was examined histologically within the period of 6 months.
Cell differentiation in cholesterol granulomas was investigated using ddY mice
and GFP bone marrow transplanted mice. Cholesterin was embedded in mice
subcutaneously and histopathological examination was carried out in a period of 6
months. Results showed that at 2 weeks, cholesterin was replaced partly by
granulation tissues. The majority of cells in the granulation tissues were
macrophages and foreign body giant cells and the center consists of small amount
of fibroblasts, collagen fibers and capillaries. At 3 months, more granulation
tissue was observed compared to 2 weeks. Similar cells were observed, however,
there were more fibroblasts, collagen bundles and capillaries present compared to
2 weeks. At 6 months, the cholesterin was mostly substituted by fibrous tissues
consisting mainly of fibroblasts and collagen fibers with some macrophages and
foreign body giant cells. Specifically, the outer part of the tissue consists of
fibroblasts, collagen bundles and capillaries and the inner portion is filled
with collagen bundles. Immunohistochemistry revealed that macrophages and foreign
body giant cells were positive to GFP and CD68 although the fibroblasts and
capillaries in the outer portion of cholesterol granulomas were GFP negative.
Some spindle shape fibroblasts were also GFP positive. Immunofluorescent double
staining revealed that cells lining the blood vessels were both positive to GFP
and CD31 indicating that those were endothelial cells and were actually derived
from the transplanted bone marrow cells. The results suggest that macrophages,
foreign body giant cells as well as fibroblasts and capillary endothelial cells
are bone marrow derived mesenchymal cells.
free
free
free
