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10.3390/ijerph13020193 http://scihub22266oqcxt.onion/10.3390/ijerph13020193 C4772213!4772213!26861363     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Environ+Res+Public+Health 2016 ; 13 (2): ä Nephropedia Template TP {{tp|p=26861363|t=2016. Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology |pdf=|usr=}}{{26861363}} gab.com Text Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology JO: Int J Environ Res Public Health http://www.kidney.de/pget.php?&tw=1&pmid=26861363 #FOAvip Early diagnosis and treatment are crucial for the outcome of central nervous system (CNS) infections. In this study, we developed a multiplex PCR-Luminex assay for the simultaneous detection of five major pathogens, including Mycobacterium tuberculosis, Cryptococcus neoformans, Streptococcus pneumoniae, and herpes simplex virus types 1 and 2, which frequently cause CNS infections. Through the hybridization reaction between multiplex PCR-amplified targets and oligonucleotide ?anti-TAG? sequences, we found that the PCR-Luminex assay could detect as low as 101?102 copies of synthetic pathogen DNAs. Furthermore, 163 cerebrospinal fluid (CSF) specimens from patients with suspected CNS infections were used to evaluate the efficiency of this multiplex PCR-Luminex method. Compared with Ziehl-Neelsen stain, this assay showed a high diagnostic accuracy for tuberculosis meningitis (sensitivity, 90.7% and specificity, 99.1%). For cryptococcal meningitis, the sensitivity and specificity were 92% and 97.1%, respectively, compared with the May Grunwald Giemsa (MGG) stain. For herpes simplex virus types 1 and 2 encephalitis, the sensitivities were 80.8% and 100%, and the specificities were 94.2% and 99%, respectively, compared with Enzyme Linked Immunosorbent Assay (ELISA) assays. Taken together, this multiplex PCR-Luminex assay showed potential efficiency for the simultaneous detection of five pathogens and may be a promising supplement to conventional methods for diagnosing CNS infections. Twit Text FOAVip Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology ????Int J Environ Res Public Health http://www.kidney.de/pget.php?&tw=1&pmid=26861363 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26861363 #FOAvip English Wikipedia <ref>{{Cite pmid|26861363}}</ref> Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology #MMPMID26861363 Zhou L; Wu R; Shi X; Feng D; Feng G; Yang Y; Dai W; Bian T; Liu T; He Y; Shi M; Zhao G Int J Environ Res Public Health 2016[Feb]; 13 (2): ä PMID26861363show ga Early diagnosis and treatment are crucial for the outcome of central nervous system (CNS) infections. In this study, we developed a multiplex PCR-Luminex assay for the simultaneous detection of five major pathogens, including Mycobacterium tuberculosis, Cryptococcus neoformans, Streptococcus pneumoniae, and herpes simplex virus types 1 and 2, which frequently cause CNS infections. Through the hybridization reaction between multiplex PCR-amplified targets and oligonucleotide ?anti-TAG? sequences, we found that the PCR-Luminex assay could detect as low as 101?102 copies of synthetic pathogen DNAs. Furthermore, 163 cerebrospinal fluid (CSF) specimens from patients with suspected CNS infections were used to evaluate the efficiency of this multiplex PCR-Luminex method. Compared with Ziehl-Neelsen stain, this assay showed a high diagnostic accuracy for tuberculosis meningitis (sensitivity, 90.7% and specificity, 99.1%). For cryptococcal meningitis, the sensitivity and specificity were 92% and 97.1%, respectively, compared with the May Grunwald Giemsa (MGG) stain. For herpes simplex virus types 1 and 2 encephalitis, the sensitivities were 80.8% and 100%, and the specificities were 94.2% and 99%, respectively, compared with Enzyme Linked Immunosorbent Assay (ELISA) assays. Taken together, this multiplex PCR-Luminex assay showed potential efficiency for the simultaneous detection of five pathogens and may be a promising supplement to conventional methods for diagnosing CNS infections. äSimultaneous Detection of Five Pathogens from Cerebrospinal Fluid Spec(epmc).pdf DeepDyve Pubget Overpricing