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10.1101/gr.199588.115 http://scihub22266oqcxt.onion/10.1101/gr.199588.115 C4772022!4772022!26786045     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Res 2016 ; 26 (3): 406-15 Nephropedia Template TP {{tp|p=26786045|t=2016. Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq |pdf=|usr=}}{{26786045}} gab.com Text Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq JO: Genome Res http://www.kidney.de/pget.php?&tw=1&pmid=26786045 #FOAvip We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human genomic DNA was digested using multiple sgRNAs combined with the Cas9 protein and then subjected to whole-genome sequencing. In vitro cleavage patterns, characteristic of on- and off-target sites, were computationally identified across the genome using a new DNA cleavage scoring system. We found that many false-positive, bulge-type off-target sites were cleaved by sgRNAs transcribed from an oligonucleotide duplex but not by those transcribed from a plasmid template. Multiplex Digenome-seq captured many bona fide off-target sites, missed by other genome-wide methods, at which indels were induced at frequencies <0.1%. After analyzing 964 sites cleaved in vitro by these sgRNAs and measuring indel frequencies at hundreds of off-target sites in cells, we propose a guideline for the choice of target sites for minimizing CRISPR-Cas9 off-target effects in the human genome. Twit Text FOAVip Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq ????Genome Res http://www.kidney.de/pget.php?&tw=1&pmid=26786045 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26786045 #FOAvip English Wikipedia <ref>{{Cite pmid|26786045}}</ref> Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq #MMPMID26786045 Kim D; Kim S; Kim S; Park J; Kim JS Genome Res 2016[Mar]; 26 (3): 406-15 PMID26786045show ga We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human genomic DNA was digested using multiple sgRNAs combined with the Cas9 protein and then subjected to whole-genome sequencing. In vitro cleavage patterns, characteristic of on- and off-target sites, were computationally identified across the genome using a new DNA cleavage scoring system. We found that many false-positive, bulge-type off-target sites were cleaved by sgRNAs transcribed from an oligonucleotide duplex but not by those transcribed from a plasmid template. Multiplex Digenome-seq captured many bona fide off-target sites, missed by other genome-wide methods, at which indels were induced at frequencies <0.1%. After analyzing 964 sites cleaved in vitro by these sgRNAs and measuring indel frequencies at hundreds of off-target sites in cells, we propose a guideline for the choice of target sites for minimizing CRISPR-Cas9 off-target effects in the human genome. äGenome-wide target specificities of CRISPR-Cas9 nucleases revealed by (epmc).pdf DeepDyve Pubget Overpricing