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2016 ; 10
(ä): 65
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Sexual Stimulation Increases the Survival of New Cells in the Accessory Olfactory
Bulb of the Male Rat
#MMPMID26973447
Unda NM
; Portillo W
; Corona R
; Paredes RG
Front Neurosci
2016[]; 10
(ä): 65
PMID26973447
show ga
Sexual behavior in rodents is modulated by the olfactory system. The olfactory
bulb (OB) is a structure that undergoes continues neurogenesis in adulthood. We
have previously shown that 15 days after males rats pace the sexual interaction
and ejaculate 1 or 3 times, there is an increase in the density of new cells that
reach the accessory olfactory bulb (AOB). The aim of the present study was to
evaluate if sexual behavior in male rats increases the density of new neurons
that survive 45 days after sexual behavior in the AOB and in the main OB (MOB).
Male rats were randomly divided in four groups: (1) Control (Ctr), males without
sexual interaction; (2) Exposed (Exp), males only exposed to a sexually receptive
female; (3) No pacing (NP), males that mated in conditions in which the female
paced the sexual interaction; (4) One ejaculation (1E), males that paced the
sexual interaction with a receptive female and ejaculated once; and (5) Three
ejaculations (3E), males that paced the sexual interaction and were allowed to
ejaculate three times. All males were injected with the DNA synthesis marker
5-bromo-2-deoxyuridine (BrdU), and were tested in one of the above conditions. 45
days later they were sacrificed, and the OBs were processed to identify new cells
and evaluate if they had differentiated into neurons. Our data indicate that
males that ejaculated three times showed an increase in the density of new cells
that survive in the posterior part of the granular cell layer of the AOB and have
more new neurons that the control group. However, no significant differences were
found in the percentage of new cells that differentiate into neurons. No
significant increase in the density of new cells was observed in the MOB. Our
data show that pacing the sexual interaction until three ejaculations increases
the density of new cells and neurons in the granular layer of the AOB, confirming
that sexual behavior induces long-lasting plastic changes in the OB.