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10.1364/BOE.7.000324

http://scihub22266oqcxt.onion/10.1364/BOE.7.000324
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C4771452!4771452!26977343
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suck abstract from ncbi


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pmid26977343      Biomed+Opt+Express 2016 ; 7 (2): 324-34
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  • Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser #MMPMID26977343
  • Perillo EP; McCracken JE; Fernée DC; Goldak JR; Medina FA; Miller DR; Yeh HC; Dunn AK
  • Biomed Opt Express 2016[Feb]; 7 (2): 324-34 PMID26977343show ga
  • Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser emits at a wavelength of 1060 nm with a usable average power of 1 W at a repetition rate of 40 MHz and a compressed pulse width of 81 fs at the sample. The laser is used to obtain deep in vivo two-color images of layer-V pyramidal neurons expressing YFP and vasculature labelled with Texas Red at depths up to 900 µm. The sub-1 µm features of dendritic spines can be resolved at a 200 µm depth.
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