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10.1364/BOE.7.000302 http://scihub22266oqcxt.onion/10.1364/BOE.7.000302 C4771450!4771450!26977341     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Opt+Express 2016 ; 7 (2): 302-11 Nephropedia Template TP {{tp|p=26977341|t=2016. Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease |pdf=|usr=}}{{26977341}} gab.com Text Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease JO: Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=26977341 #FOAvip A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease. Twit Text FOAVip Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease ????Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=26977341 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26977341 #FOAvip English Wikipedia <ref>{{Cite pmid|26977341}}</ref> Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease #MMPMID26977341 Pullman JM; Nylk J; Campbell EC; Gunn-Moore FJ; Prystowsky MB; Dholakia K Biomed Opt Express 2016[Feb]; 7 (2): 302-11 PMID26977341show ga A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease. äVisualization of podocyte substructure with structured illumination mi(epmc).pdf DeepDyve Pubget Overpricing