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Genotyping of Cryptosporidium Species and Their Clinical Manifestations in
Patients with Renal Transplantation and Human Immunodeficiency Virus Infection
#MMPMID26981284
Dey A
; Ghoshal U
; Agarwal V
; Ghoshal UC
J Pathog
2016[]; 2016
(?): 2623602
PMID26981284
show ga
In the present study we aimed to determine (i) frequency of Cryptosporidium
species among patients with renal transplantation (RT) and human immunodeficiency
virus (HIV) infection and (ii) relationship of the nature, severity, and duration
of symptoms with different species and load of Cryptosporidium. Stool samples
from 70 (42 RT and 28 HIV) and 140 immunocompromised patients with and without
cryptosporidiosis by modified Kinyoun's staining were subjected to qPCR-melting
curve analysis for identification of parasite species. qPCR detected one
microscopically negative sample to be positive for cryptosporidiosis. C. hominis,
C. parvum, and mixed infection were detected in 50/71 (70.4%), 19/71 (26.8%), and
2/71 (2.8%) patients, respectively. Patients with cryptosporidiosis had higher
stool frequency (median, IQR: 4, 3-6/d versus 3, 2-4/d; P = 0.017) and watery
stool (52/71 [73%] versus 64/139 [46%]; P = 0.003). Parasite load (median, IQR:
Log10 6.37 (5.65-7.12), Log10 5.81 (4.26-6.65); P = 0.046) and nausea/vomiting
(29/50 [58%] versus 5/19 [26%]; P = 0.032) were more frequent with C. hominis
than with C. parvum infection. Thus, Cryptosporidium spp. (mainly C. hominis) is
a common cause of diarrhoea in RT and HIV patients.
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