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10.1038/oncsis.2015.24 http://scihub22266oqcxt.onion/10.1038/oncsis.2015.24 C4767936!4767936!26344692     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Oncogenesis 2015 ; 4 (9): e165- Nephropedia Template TP {{tp|p=26344692|t=2015. Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression |pdf=|usr=}}{{26344692}} gab.com Text Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression JO: Oncogenesis http://www.kidney.de/pget.php?&tw=1&pmid=26344692 #FOAvip Abnormally stiff substrates have been shown to trigger cancer progression. However, the detailed molecular mechanisms underlying this trigger are not clear. In this study, we cultured T84 human colorectal cancer cells on plastic dishes to create a stiff substrate or on collagen-I gel to create a soft substrate. The stiff substrate enhanced the expression of matrix metalloproteinase-7 (MMP-7), an indicator of poor prognosis. In addition, we used polyacrylamide gels (2, 67 and 126?kPa) so that the MMP-7 expression on the 126-kPa gel was higher compared with that on the 2-kPa gel. Next, we investigated whether yes-associated protein (YAP) affected the MMP-7 expression. YAP knockdown decreased MMP-7 expression. Treatment with inhibitors of epidermal growth factor receptor (EGFR) and myosin regulatory light chain (MRLC) and integrin-?2 or integrin-?1 knockdown downregulated MMP-7 expression. Finally, we demonstrated that YAP, EGFR, integrin-?2?1 and MRLC produced a positive feedback loop that enhanced MMP-7 expression. These findings suggest that stiff substrates enhanced colorectal cancer cell viability by upregulating MMP-7 expression through a positive feedback loop. Twit Text FOAVip Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression ????Oncogenesis http://www.kidney.de/pget.php?&tw=1&pmid=26344692 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26344692 #FOAvip English Wikipedia <ref>{{Cite pmid|26344692}}</ref> Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression #MMPMID26344692 Nukuda A; Sasaki C; Ishihara S; Mizutani T; Nakamura K; Ayabe T; Kawabata K; Haga H Oncogenesis 2015[Sep]; 4 (9): e165- PMID26344692show ga Abnormally stiff substrates have been shown to trigger cancer progression. However, the detailed molecular mechanisms underlying this trigger are not clear. In this study, we cultured T84 human colorectal cancer cells on plastic dishes to create a stiff substrate or on collagen-I gel to create a soft substrate. The stiff substrate enhanced the expression of matrix metalloproteinase-7 (MMP-7), an indicator of poor prognosis. In addition, we used polyacrylamide gels (2, 67 and 126?kPa) so that the MMP-7 expression on the 126-kPa gel was higher compared with that on the 2-kPa gel. Next, we investigated whether yes-associated protein (YAP) affected the MMP-7 expression. YAP knockdown decreased MMP-7 expression. Treatment with inhibitors of epidermal growth factor receptor (EGFR) and myosin regulatory light chain (MRLC) and integrin-?2 or integrin-?1 knockdown downregulated MMP-7 expression. Finally, we demonstrated that YAP, EGFR, integrin-?2?1 and MRLC produced a positive feedback loop that enhanced MMP-7 expression. These findings suggest that stiff substrates enhanced colorectal cancer cell viability by upregulating MMP-7 expression through a positive feedback loop. äStiff substrates increase YAP-signaling-mediated matrix metalloprotein(epmc).pdf DeepDyve Pubget Overpricing