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10.1194/jlr.M064923 http://scihub22266oqcxt.onion/10.1194/jlr.M064923 C4766994!4766994 !26724485     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26724485 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Lipid+Res 2016 ; 57 (3 ): 451-63 Nephropedia Template TP {{tp|p=26724485 |t=2016. Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ?-glucosidases |pdf=|usr=}}{{26724485 }} gab.com Text Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular -glucosidases JO: J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=26724485 #FOAvip The membrane lipid glucosylceramide (GlcCer) is continuously formed and degraded. Cells express two GlcCer-degrading ?-glucosidases, glucocerebrosidase (GBA) and GBA2, located in and outside the lysosome, respectively. Here we demonstrate that through transglucosylation both GBA and GBA2 are able to catalyze in vitro the transfer of glucosyl-moieties from GlcCer to cholesterol, and vice versa. Furthermore, the natural occurrence of 1-O-cholesteryl-?-D-glucopyranoside (GlcChol) in mouse tissues and human plasma is demonstrated using LC-MS/MS and (13)C6-labeled GlcChol as internal standard. In cells, the inhibition of GBA increases GlcChol, whereas inhibition of GBA2 decreases glucosylated sterol. Similarly, in GBA2-deficient mice, GlcChol is reduced. Depletion of GlcCer by inhibition of GlcCer synthase decreases GlcChol in cells and likewise in plasma of inhibitor-treated Gaucher disease patients. In tissues of mice with Niemann-Pick type C disease, a condition characterized by intralysosomal accumulation of cholesterol, marked elevations in GlcChol occur as well. When lysosomal accumulation of cholesterol is induced in cultured cells, GlcChol is formed via lysosomal GBA. This illustrates that reversible transglucosylation reactions are highly dependent on local availability of suitable acceptors. In conclusion, mammalian tissues contain GlcChol formed by transglucosylation through ?-glucosidases using GlcCer as donor. Our findings reveal a novel metabolic function for GlcCer. Twit Text FOAVip Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular -glucosidases ????J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=26724485 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26724485 #FOAvip English Wikipedia <ref>{{Cite pmid|26724485 }}</ref> Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ?-glucosidases #MMPMID26724485 Marques AR ; Mirzaian M ; Akiyama H ; Wisse P ; Ferraz MJ ; Gaspar P ; Ghauharali-van der Vlugt K ; Meijer R ; Giraldo P ; Alfonso P ; Irún P ; Dahl M ; Karlsson S ; Pavlova EV ; Cox TM ; Scheij S ; Verhoek M ; Ottenhoff R ; van Roomen CP ; Pannu NS ; van Eijk M ; Dekker N ; Boot RG ; Overkleeft HS ; Blommaart E ; Hirabayashi Y ; Aerts JM J Lipid Res 2016[Mar]; 57 (3 ): 451-63 PMID26724485 show ga The membrane lipid glucosylceramide (GlcCer) is continuously formed and degraded. Cells express two GlcCer-degrading ?-glucosidases, glucocerebrosidase (GBA) and GBA2, located in and outside the lysosome, respectively. Here we demonstrate that through transglucosylation both GBA and GBA2 are able to catalyze in vitro the transfer of glucosyl-moieties from GlcCer to cholesterol, and vice versa. Furthermore, the natural occurrence of 1-O-cholesteryl-?-D-glucopyranoside (GlcChol) in mouse tissues and human plasma is demonstrated using LC-MS/MS and (13)C6-labeled GlcChol as internal standard. In cells, the inhibition of GBA increases GlcChol, whereas inhibition of GBA2 decreases glucosylated sterol. Similarly, in GBA2-deficient mice, GlcChol is reduced. Depletion of GlcCer by inhibition of GlcCer synthase decreases GlcChol in cells and likewise in plasma of inhibitor-treated Gaucher disease patients. In tissues of mice with Niemann-Pick type C disease, a condition characterized by intralysosomal accumulation of cholesterol, marked elevations in GlcChol occur as well. When lysosomal accumulation of cholesterol is induced in cultured cells, GlcChol is formed via lysosomal GBA. This illustrates that reversible transglucosylation reactions are highly dependent on local availability of suitable acceptors. In conclusion, mammalian tissues contain GlcChol formed by transglucosylation through ?-glucosidases using GlcCer as donor. Our findings reveal a novel metabolic function for GlcCer. |Animals [MESH] |COS Cells [MESH] |Chlorocebus aethiops [MESH] |Cholesterol/*metabolism [MESH] |Female [MESH] |Gaucher Disease/metabolism [MESH] |Glycosylation [MESH] |Humans [MESH] |Male [MESH] |Mice [MESH] |Niemann-Pick Diseases/metabolism [MESH] |RAW 264.7 Cells [MESH]Glucosylated cholesterol in mammalian cells and tissues: formation and(epmc).pdf DeepDyve Pubget Overpricing