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10.1016/j.diagmicrobio.2015.11.017

http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2015.11.017
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suck abstract from ncbi


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pmid26711635
      Diagn+Microbiol+Infect+Dis 2016 ; 84 (3 ): 268-73
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  • Diagnostic performance of a multiplex PCR assay for meningitis in an HIV-infected population in Uganda #MMPMID26711635
  • Rhein J ; Bahr NC ; Hemmert AC ; Cloud JL ; Bellamkonda S ; Oswald C ; Lo E ; Nabeta H ; Kiggundu R ; Akampurira A ; Musubire A ; Williams DA ; Meya DB ; Boulware DR
  • Diagn Microbiol Infect Dis 2016[Mar]; 84 (3 ): 268-73 PMID26711635 show ga
  • Meningitis remains a worldwide problem, and rapid diagnosis is essential to optimize survival. We evaluated the utility of a multiplex PCR test in differentiating possible etiologies of meningitis. Cerebrospinal fluid (CSF) from 69 HIV-infected Ugandan adults with meningitis was collected at diagnosis (n=51) and among persons with cryptococcal meningitis during therapeutic lumbar punctures (n=68). Cryopreserved CSF specimens were analyzed with BioFire FilmArray® Meningitis/Encephalitis panel, which targets 17 pathogens. The panel detected Cryptococcus in the CSF of patients diagnosed with a first episode of cryptococcal meningitis by fungal culture with 100% sensitivity and specificity and differentiated between fungal relapse and paradoxical immune reconstitution inflammatory syndrome in recurrent episodes. A negative FilmArray result was predictive of CSF sterility on follow-up lumbar punctures for cryptococcal meningitis. EBV was frequently detected in this immunosuppressed population (n=45). Other pathogens detected included: cytomegalovirus (n=2), varicella zoster virus (n=2), human herpes virus 6 (n=1), and Streptococcus pneumoniae (n=1). The FilmArray Meningitis/Encephalitis panel offers a promising platform for rapid meningitis diagnosis.
  • |*AIDS-Related Opportunistic Infections [MESH]
  • |*Multiplex Polymerase Chain Reaction/methods/standards [MESH]
  • |Adult [MESH]
  • |CD4 Lymphocyte Count [MESH]
  • |Cohort Studies [MESH]
  • |Female [MESH]
  • |Humans [MESH]
  • |Immunocompromised Host [MESH]
  • |Male [MESH]
  • |Meningitis, Cryptococcal/diagnosis/microbiology [MESH]
  • |Meningitis/*diagnosis/*microbiology [MESH]
  • |Prospective Studies [MESH]
  • |Reproducibility of Results [MESH]
  • |Sensitivity and Specificity [MESH]


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