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10.2337/db15-1264 http://scihub22266oqcxt.onion/10.2337/db15-1264 C4764152!4764152!26647386     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26647386&cmd=llinks): Failed to open stream: HTTP request failed! 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Monitoring C-Peptide Storage and Secretion in Islet ?-Cells In Vitro and In Vivo |pdf=|usr=}}{{26647386}} gab.com Text Monitoring C-Peptide Storage and Secretion in Islet -Cells In Vitro and In Vivo JO: Diabetes http://www.kidney.de/pget.php?&tw=1&pmid=26647386 #FOAvip Human proinsulin with C-peptide?bearing Superfolder Green Fluorescent Protein (CpepSfGFP) has been expressed in transgenic mice, driven by the Ins1 promoter. The protein, expressed exclusively in ?-cells, is processed and stored as CpepSfGFP and human insulin comprising only ?0.04% of total islet proinsulin plus insulin, exerting no metabolic impact. The kinetics of the release of insulin and CpepSfGFP from isolated islets appear identical. Upon a single acute stimulatory challenge in vitro, fractional release of insulin does not detectably deplete islet fluorescence. In vivo, fluorescence imaging of the pancreatic surface allows, for the first time, visual assessment of pancreatic islet insulin content, and we demonstrate that CpepSfGFP visibly declines upon diabetes progression in live lepRdb/db mice. In anesthetized mice, after intragastric or intravenous saline delivery, pancreatic CpepSfGFP (insulin) content remains undiminished. Remarkably, however, within 20 min after acute intragastric or intravenous glucose delivery (with blood glucose concentrations reaching >15 mmol/L), a small subset of islets shows rapid dispossession of a major fraction of their stored CpepSfGFP (insulin) content, whereas most islets exhibit no demonstrable loss of CpepSfGFP (insulin). These studies strongly suggest that there are ?first responder? islets to an in vivo glycemic challenge, which cannot be replicated by islets in vitro. Twit Text FOAVip Monitoring C-Peptide Storage and Secretion in Islet -Cells In Vitro and In Vivo ????Diabetes http://www.kidney.de/pget.php?&tw=1&pmid=26647386 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26647386 #FOAvip English Wikipedia <ref>{{Cite pmid|26647386}}</ref> Monitoring C-Peptide Storage and Secretion in Islet ?-Cells In Vitro and In Vivo #MMPMID26647386 Zhu S; Larkin D; Lu S; Inouye C; Haataja L; Anjum A; Kennedy R; Castle D; Arvan P Diabetes 2016[Mar]; 65 (3): 699-709 PMID26647386show ga Human proinsulin with C-peptide?bearing Superfolder Green Fluorescent Protein (CpepSfGFP) has been expressed in transgenic mice, driven by the Ins1 promoter. The protein, expressed exclusively in ?-cells, is processed and stored as CpepSfGFP and human insulin comprising only ?0.04% of total islet proinsulin plus insulin, exerting no metabolic impact. The kinetics of the release of insulin and CpepSfGFP from isolated islets appear identical. Upon a single acute stimulatory challenge in vitro, fractional release of insulin does not detectably deplete islet fluorescence. In vivo, fluorescence imaging of the pancreatic surface allows, for the first time, visual assessment of pancreatic islet insulin content, and we demonstrate that CpepSfGFP visibly declines upon diabetes progression in live lepRdb/db mice. In anesthetized mice, after intragastric or intravenous saline delivery, pancreatic CpepSfGFP (insulin) content remains undiminished. Remarkably, however, within 20 min after acute intragastric or intravenous glucose delivery (with blood glucose concentrations reaching >15 mmol/L), a small subset of islets shows rapid dispossession of a major fraction of their stored CpepSfGFP (insulin) content, whereas most islets exhibit no demonstrable loss of CpepSfGFP (insulin). These studies strongly suggest that there are ?first responder? islets to an in vivo glycemic challenge, which cannot be replicated by islets in vitro. äMonitoring C-Peptide Storage and Secretion in Islet ?-Cells In Vitro a(epmc).pdf DeepDyve Pubget Overpricing