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10.1038/srep21809 http://scihub22266oqcxt.onion/10.1038/srep21809 C4763179!4763179!26902619     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=26902619|t=2016. Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation |pdf=|usr=}}{{26902619}} gab.com Text Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26902619 #FOAvip Lactococcus lactis, a non-pathogenic bacteria, has been genetically engineered to express the III7?10 fragment of human fibronectin as a membrane protein. The engineered L. lactis is able to develop biofilms on different surfaces (such as glass and synthetic polymers) and serves as a long-term substrate for mammalian cell culture, specifically human mesenchymal stem cells (hMSC). This system constitutes a living interface between biomaterials and stem cells. The engineered biofilms remain stable and viable for up to 28 days while the expressed fibronectin fragment induces hMSC adhesion. We have optimised conditions to allow long-term mammalian cell culture, and found that the biofilm is functionally equivalent to a fibronectin-coated surface in terms of osteoblastic differentiation using bone morphogenetic protein 2 (BMP-2) added to the medium. This living bacteria interface holds promise as a dynamic substrate for stem cell differentiation that can be further engineered to express other biochemical cues to control hMSC differentiation. Twit Text FOAVip Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26902619 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26902619 #FOAvip English Wikipedia <ref>{{Cite pmid|26902619}}</ref> Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation #MMPMID26902619 Hay JJ; Rodrigo-Navarro A; Hassi K; Moulisova V; Dalby MJ; Salmeron-Sanchez M Sci Rep 2016[]; 6 (ä): ä PMID26902619show ga Lactococcus lactis, a non-pathogenic bacteria, has been genetically engineered to express the III7?10 fragment of human fibronectin as a membrane protein. The engineered L. lactis is able to develop biofilms on different surfaces (such as glass and synthetic polymers) and serves as a long-term substrate for mammalian cell culture, specifically human mesenchymal stem cells (hMSC). This system constitutes a living interface between biomaterials and stem cells. The engineered biofilms remain stable and viable for up to 28 days while the expressed fibronectin fragment induces hMSC adhesion. We have optimised conditions to allow long-term mammalian cell culture, and found that the biofilm is functionally equivalent to a fibronectin-coated surface in terms of osteoblastic differentiation using bone morphogenetic protein 2 (BMP-2) added to the medium. This living bacteria interface holds promise as a dynamic substrate for stem cell differentiation that can be further engineered to express other biochemical cues to control hMSC differentiation. äLiving biointerfaces based on non-pathogenic bacteria support stem cel(epmc).pdf DeepDyve Pubget Overpricing