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10.1038/ncomms10536 http://scihub22266oqcxt.onion/10.1038/ncomms10536 C4762882!4762882!26892726     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26892726&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26892726|t=2016. Whole-genome mutational burden analysis of three pluripotency induction methods |pdf=|usr=}}{{26892726}} gab.com Text Whole-genome mutational burden analysis of three pluripotency induction methods JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26892726 #FOAvip There is concern that the stresses of inducing pluripotency may lead to deleterious DNA mutations in induced pluripotent stem cell (iPSC) lines, which would compromise their use for cell therapies. Here we report comparative genomic analysis of nine isogenic iPSC lines generated using three reprogramming methods: integrating retroviral vectors, non-integrating Sendai virus and synthetic mRNAs. We used whole-genome sequencing and de novo genome mapping to identify single-nucleotide variants, insertions and deletions, and structural variants. Our results show a moderate number of variants in the iPSCs that were not evident in the parental fibroblasts, which may result from reprogramming. There were only small differences in the total numbers and types of variants among different reprogramming methods. Most importantly, a thorough genomic analysis showed that the variants were generally benign. We conclude that the process of reprogramming is unlikely to introduce variants that would make the cells inappropriate for therapy. Twit Text FOAVip Whole-genome mutational burden analysis of three pluripotency induction methods ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26892726 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26892726 #FOAvip English Wikipedia <ref>{{Cite pmid|26892726}}</ref> Whole-genome mutational burden analysis of three pluripotency induction methods #MMPMID26892726 Bhutani K; Nazor KL; Williams R; Tran H; Dai H; D?akula ?; Cho EH; Pang AWC; Rao M; Cao H; Schork NJ; Loring JF Nat Commun 2016[]; 7 (ä): ä PMID26892726show ga There is concern that the stresses of inducing pluripotency may lead to deleterious DNA mutations in induced pluripotent stem cell (iPSC) lines, which would compromise their use for cell therapies. Here we report comparative genomic analysis of nine isogenic iPSC lines generated using three reprogramming methods: integrating retroviral vectors, non-integrating Sendai virus and synthetic mRNAs. We used whole-genome sequencing and de novo genome mapping to identify single-nucleotide variants, insertions and deletions, and structural variants. Our results show a moderate number of variants in the iPSCs that were not evident in the parental fibroblasts, which may result from reprogramming. There were only small differences in the total numbers and types of variants among different reprogramming methods. Most importantly, a thorough genomic analysis showed that the variants were generally benign. We conclude that the process of reprogramming is unlikely to introduce variants that would make the cells inappropriate for therapy. äWhole-genome mutational burden analysis of three pluripotency inductio(epmc).pdf DeepDyve Pubget Overpricing