Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/anie.201510847 http://scihub22266oqcxt.onion/10.1002/anie.201510847 C4762321!4762321!26833452     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26833452&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Angew+Chem+Int+Ed+Engl 2016 ; 55 (9): 3224-8 Nephropedia Template TP {{tp|p=26833452|t=2016. RNA Hairpin Folding in the Crowded Cell |pdf=|usr=}}{{26833452}} gab.com Text RNA Hairpin Folding in the Crowded Cell JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26833452 #FOAvip Precise secondary and tertiary structure formation is critically important for the cellular functionality of ribonucleic acids (RNAs). RNA folding studies were mainly conducted in vitro, without the possibility of validating these experiments inside cells. Here, we directly resolve the folding stability of a hairpin?structured RNA inside live mammalian cells. We find that the stability inside the cell is comparable to that in dilute physiological buffer. On the contrary, the addition of in vitro artificial crowding agents, with the exception of high?molecular?weight PEG, leads to a destabilization of the hairpin structure through surface interactions and reduction in water activity. We further show that RNA stability is highly variable within cell populations as well as within subcellular regions of the cytosol and nucleus. We conclude that inside cells the RNA is subject to (localized) stabilizing and destabilizing effects that lead to an on average only marginal modulation compared to diluted buffer. Twit Text FOAVip RNA Hairpin Folding in the Crowded Cell ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26833452 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26833452 #FOAvip English Wikipedia <ref>{{Cite pmid|26833452}}</ref> RNA Hairpin Folding in the Crowded Cell #MMPMID26833452 Gao M; Gnutt D; Orban A; Appel B; Righetti F; Winter R; Narberhaus F; Müller S; Ebbinghaus S Angew Chem Int Ed Engl 2016[Feb]; 55 (9): 3224-8 PMID26833452show ga Precise secondary and tertiary structure formation is critically important for the cellular functionality of ribonucleic acids (RNAs). RNA folding studies were mainly conducted in vitro, without the possibility of validating these experiments inside cells. Here, we directly resolve the folding stability of a hairpin?structured RNA inside live mammalian cells. We find that the stability inside the cell is comparable to that in dilute physiological buffer. On the contrary, the addition of in vitro artificial crowding agents, with the exception of high?molecular?weight PEG, leads to a destabilization of the hairpin structure through surface interactions and reduction in water activity. We further show that RNA stability is highly variable within cell populations as well as within subcellular regions of the cytosol and nucleus. We conclude that inside cells the RNA is subject to (localized) stabilizing and destabilizing effects that lead to an on average only marginal modulation compared to diluted buffer. äRNA Hairpin Folding in the Crowded Cell (epmc).pdf DeepDyve Pubget Overpricing