Protection against ischemia/reperfusion?induced renal injury by co?treatment with
erythropoietin and sodium selenite
#MMPMID26647839
Liu L
; Liu C
; Hou L
; Lv J
; Wu F
; Yang X
; Ren S
; Ji W
; Wang M
; Chen L
Mol Med Rep
2015[Dec]; 12
(6
): 7933-40
PMID26647839
show ga
Ischemia/reperfusion injury (IRI) has lzong been an area of concern and focus of
investigations. Erythropoietin (EPO) exhibits multiple protective effects, and
selenium is an antioxidant trace element in the body, however, there have been no
reports concerning the effects of EPO combined with sodium selenite on IRI. In
the present study, a mouse model of renal IRI (RIRI) was pre?treated with EPO and
sodium selenite to determine the most appropriate combination ratio of the two
for further investigation. The results revealed that EPO and sodium selenite had
synergistic protective effects in RIRI. EPO was identified as the predominant
treatment component, with sodium selenite serving as an adjuvant, and combination
treatment was markedly more effective, compared with treatment with either drug
alone. The optimal ratio of treatment was 10:1 (10 IU EPO: 1 µg sodium selenite).
The results indicated that RIRI markedly induced renal injury, as evidenced by
elevated levels of blood urea nitrogen (BUN), as well as higher pathological
scores, based on hematoxylin and eosin staining. Pre?treatment with EPO and
sodium selenite significantly decreased serum expression levels of BUN and
malonaldehyde, and increased the expression levels of superoxide dismutase,
glutathione peroxidase and nitric oxide (NO), compared with the model group.
Furthermore, co?treatment with EPO and sodium selenite upregulated the protein
expression levels of phosphatidylinositol?3 kinase (PI3K) in renal tissue
samples. Together, the results suggested that co?administration of EPO and sodium
selenite effectively ameliorates IRI?induced renal injury by reducing oxidative
stress and activating the PI3K/NO signaling pathway.
|Animals
[MESH]
|Blood Urea Nitrogen
[MESH]
|Drug Synergism
[MESH]
|Drug Therapy, Combination
[MESH]
|Erythropoietin/administration & dosage/*therapeutic use
[MESH]
|Glutathione Peroxidase/metabolism
[MESH]
|Immunohistochemistry
[MESH]
|Kidney/metabolism/pathology
[MESH]
|Male
[MESH]
|Mice
[MESH]
|Mice, Inbred Strains
[MESH]
|Nitric Oxide/metabolism
[MESH]
|Oxidative Stress/drug effects
[MESH]
|Phosphatidylinositol 3-Kinase/metabolism
[MESH]
|Protective Agents/administration & dosage/*therapeutic use
[MESH]
|Reperfusion Injury/*prevention & control
[MESH]
|Sodium Selenite/administration & dosage/*therapeutic use
[MESH]
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