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10.1093/bioinformatics/btv423 http://scihub22266oqcxt.onion/10.1093/bioinformatics/btv423 C4757951!4757951!26209430     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioinformatics 2015 ; 31 (22): 3676-8 Nephropedia Template TP {{tp|p=26209430|t=2015. CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation |pdf=|usr=}}{{26209430}} gab.com Text CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation JO: Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=26209430 #FOAvip Summary: The CRISPR/Cas9 system was recently developed as a powerful and flexible technology for targeted genome engineering, including genome editing (altering the genetic sequence) and gene regulation (without altering the genetic sequence). These applications require the design of single guide RNAs (sgRNAs) that are efficient and specific. However, this remains challenging, as it requires the consideration of many criteria. Several sgRNA design tools have been developed for gene editing, but currently there is no tool for the design of sgRNAs for gene regulation. With accumulating experimental data on the use of CRISPR/Cas9 for gene editing and regulation, we implement a comprehensive computational tool based on a set of sgRNA design rules summarized from these published reports. We report a genome-wide sgRNA design tool and provide an online website for predicting sgRNAs that are efficient and specific. We name the tool CRISPR-ERA, for clustered regularly interspaced short palindromic repeat-mediated editing, repression, and activation (ERA).Availability and implementation: http://CRISPR-ERA.stanford.edu.Contact: stanley.qi@stanford.edu or xwwang@tsinghua.edu.cnSupplementary information:Supplementary data are available at Bioinformatics online. Twit Text FOAVip CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation ????Bioinformatics http://www.kidney.de/pget.php?&tw=1&pmid=26209430 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26209430 #FOAvip English Wikipedia <ref>{{Cite pmid|26209430}}</ref> CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation #MMPMID26209430 Liu H; Wei Z; Dominguez A; Li Y; Wang X; Qi LS Bioinformatics 2015[Nov]; 31 (22): 3676-8 PMID26209430show ga Summary: The CRISPR/Cas9 system was recently developed as a powerful and flexible technology for targeted genome engineering, including genome editing (altering the genetic sequence) and gene regulation (without altering the genetic sequence). These applications require the design of single guide RNAs (sgRNAs) that are efficient and specific. However, this remains challenging, as it requires the consideration of many criteria. Several sgRNA design tools have been developed for gene editing, but currently there is no tool for the design of sgRNAs for gene regulation. With accumulating experimental data on the use of CRISPR/Cas9 for gene editing and regulation, we implement a comprehensive computational tool based on a set of sgRNA design rules summarized from these published reports. We report a genome-wide sgRNA design tool and provide an online website for predicting sgRNAs that are efficient and specific. We name the tool CRISPR-ERA, for clustered regularly interspaced short palindromic repeat-mediated editing, repression, and activation (ERA).Availability and implementation: http://CRISPR-ERA.stanford.edu.Contact: stanley.qi@stanford.edu or xwwang@tsinghua.edu.cnSupplementary information:Supplementary data are available at Bioinformatics online. äCRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editi(epmc).pdf DeepDyve Pubget Overpricing