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10.1038/ncomms10709 http://scihub22266oqcxt.onion/10.1038/ncomms10709 C4757758!4757758!26880188     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26880188|t=2016. Precision multidimensional assay for high-throughput microRNA drug discovery |pdf=|usr=}}{{26880188}} gab.com Text Precision multidimensional assay for high-throughput microRNA drug discovery JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26880188 #FOAvip Development of drug discovery assays that combine high content with throughput is challenging. Information-processing gene networks can address this challenge by integrating multiple potential targets of drug candidates' activities into a small number of informative readouts, reporting simultaneously on specific and non-specific effects. Here we show a family of networks implementing this concept in a cell-based drug discovery assay for miRNA drug targets. The networks comprise multiple modules reporting on specific effects towards an intended miRNA target, together with non-specific effects on gene expression, off-target miRNAs and RNA interference pathway. We validate the assays using known perturbations of on- and off-target miRNAs, and evaluate an ?700 compound library in an automated screen with a follow-up on specific and non-specific hits. We further customize and validate assays for additional drug targets and non-specific inputs. Our study offers a novel framework for precision drug discovery assays applicable to diverse target families. Twit Text FOAVip Precision multidimensional assay for high-throughput microRNA drug discovery ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26880188 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26880188 #FOAvip English Wikipedia <ref>{{Cite pmid|26880188}}</ref> Precision multidimensional assay for high-throughput microRNA drug discovery #MMPMID26880188 Haefliger B; Prochazka L; Angelici B; Benenson Y Nat Commun 2016[]; 7 (ä): ä PMID26880188show ga Development of drug discovery assays that combine high content with throughput is challenging. Information-processing gene networks can address this challenge by integrating multiple potential targets of drug candidates' activities into a small number of informative readouts, reporting simultaneously on specific and non-specific effects. Here we show a family of networks implementing this concept in a cell-based drug discovery assay for miRNA drug targets. The networks comprise multiple modules reporting on specific effects towards an intended miRNA target, together with non-specific effects on gene expression, off-target miRNAs and RNA interference pathway. We validate the assays using known perturbations of on- and off-target miRNAs, and evaluate an ?700 compound library in an automated screen with a follow-up on specific and non-specific hits. We further customize and validate assays for additional drug targets and non-specific inputs. Our study offers a novel framework for precision drug discovery assays applicable to diverse target families. äPrecision multidimensional assay for high-throughput microRNA drug dis(epmc).pdf DeepDyve Pubget Overpricing