Hydroxytyrosol Protects against Myocardial Ischemia/Reperfusion Injury through a
PI3K/Akt-Dependent Mechanism
#MMPMID26966340
Pei YH
; Chen J
; Xie L
; Cai XM
; Yang RH
; Wang X
; Gong JB
Mediators Inflamm
2016[]; 2016
(?): 1232103
PMID26966340
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OBJECTIVE: To investigate the effects and mechanisms of hydroxytyrosol (HT)
during the pathogenesis of myocardial ischemia reperfusion (I/R) in rat hearts.
METHODS: The rats were randomized into five groups: sham group, I/R group, HT+I/R
group, HT+LY294002+I/R group, and LY+I/R group. Myocardial infarct size, markers
of oxidative stress, extent of myocardial apoptosis, echocardiographically
assessed cardiac function, and expression of Akt and GSK 3? were measured in each
group. RESULTS: Prereperfusion administration of HT was associated with a
significantly smaller area of myocardial infarction and remarkably decreased
level of myocardial apoptosis and necrosis, as evidenced by a lower apoptotic
index, reduced cleaved caspase-3, and the serum activities of lactate
dehydrogenase and creatinine kinase MB. Moreover, HT also attenuated the
impairment of cardiac systolic function. However, cotreatment with LY294002 and
HT completely abolished the above cardioprotective effects of HT. A subsequent
mechanistic study revealed that the cardioprotective effects of HT during the
process of I/R of the myocardium were dependent on the activation of the
Akt/GSK3? pathway. CONCLUSION: Pretreatment with HT may have antiapoptotic and
cardioprotective effects against myocardial I/R injury, and these effects seem to
be related to the activation of the Akt/GSK3? pathway in the myocardium.
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