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10.1016/j.chembiol.2015.09.015 http://scihub22266oqcxt.onion/10.1016/j.chembiol.2015.09.015 C4756654!4756654!26548613     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chem+Biol 2015 ; 22 (11): 1521-30 Nephropedia Template TP {{tp|p=26548613|t=2015. Host-microbe protein interactions during bacterial infection |pdf=|usr=}}{{26548613}} gab.com Text Host-microbe protein interactions during bacterial infection JO: Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical cross-linking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with Acinetobacter baumannii. These efforts resulted in identification of 3076 total cross-linked peptide pairs and 46 interspecies protein-protein interactions. Most notably, the key A. baumannii virulence factor, OmpA, was identified cross-linked to host proteins involved in desmosomes, specialized structures that mediate host cell-to-cell adhesion. Co-immunoprecipitation and transposon mutant experiments were used to verify these interactions and demonstrate relevance for host cell invasion and acute murine lung infection. These results shed new light on A. baumannii-host protein interactions and their structural features and the presented approach is generally applicable to other systems. Twit Text FOAVip Host-microbe protein interactions during bacterial infection ????Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip English Wikipedia <ref>{{Cite pmid|26548613}}</ref> Host-microbe protein interactions during bacterial infection #MMPMID26548613 Schweppe DK; Harding C; Chavez JD; Wu X; Ramage E; Singh PK; Manoil C; Bruce JE Chem Biol 2015[Nov]; 22 (11): 1521-30 PMID26548613show ga Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical cross-linking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with Acinetobacter baumannii. These efforts resulted in identification of 3076 total cross-linked peptide pairs and 46 interspecies protein-protein interactions. Most notably, the key A. baumannii virulence factor, OmpA, was identified cross-linked to host proteins involved in desmosomes, specialized structures that mediate host cell-to-cell adhesion. Co-immunoprecipitation and transposon mutant experiments were used to verify these interactions and demonstrate relevance for host cell invasion and acute murine lung infection. These results shed new light on A. baumannii-host protein interactions and their structural features and the presented approach is generally applicable to other systems. äHost-microbe protein interactions during bacterial infection (epmc).pdf DeepDyve Pubget Overpricing