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2016 ; 68
(2
): 223-8
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Fibroblast sources: Where can we get them?
#MMPMID25060709
Fernandes IR
; Russo FB
; Pignatari GC
; Evangelinellis MM
; Tavolari S
; Muotri AR
; Beltrão-Braga PC
Cytotechnology
2016[Mar]; 68
(2
): 223-8
PMID25060709
show ga
Fibroblasts are cells widely used in cell culture, both for transient primary
cell culture or permanent as transformed cell lines. Lately, fibroblasts become
cell sources for use in disease modeling after cell reprogramming because it is
easily accessible in the body. Fibroblasts in patients will maintain all genetic
background during reprogramming into induced pluripotent stem cells. In spite of
their large use, fibroblasts are obtained after an invasive procedure, a
superficial punch skin biopsy, collected under patient's local anesthesia. Taking
into consideration the minimum patient's discomfort during and after the biopsy
procedure, as well as the aesthetics aspect, it is essential to reflect on the
best site of the body for the biopsy procedure combined with the success of
getting robust fibroblast cultures in the lab. For this purpose, we compared the
efficiency of four biopsy sites of the body (skin from eyelid, back of the ear,
abdominal cesarean scar and groin). Cell proliferation assays and viability after
cryopreservation were measured. Our results revealed that scar tissue provided
fibroblasts with higher proliferative rates. Also, fibroblasts from scar tissues
presented a higher viability after the thawing process.
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