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10.1038/aps.2015.114 http://scihub22266oqcxt.onion/10.1038/aps.2015.114 C4753365!4753365 !26775661     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26775661 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Acta+Pharmacol+Sin 2016 ; 37 (2 ): 235-45 Nephropedia Template TP {{tp|p=26775661 |t=2016. Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy |pdf=|usr=}}{{26775661 }} gab.com Text Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy JO: Acta Pharmacol Sin http://www.kidney.de/pget.php?&tw=1&pmid=26775661 #FOAvip AIM: A previous report shows that emodin extracted from the Chinese herbs rhubarb and giant knotweed rhizome can ameliorate the anticancer drug cisplatin-induced injury of HEK293 cells. In this study, we investigated whether and how emodin could protect renal tubular epithelial cells against cisplatin-induced nephrotoxicity in vitro. METHODS: The viability and apoptosis of normal rat renal tubular epithelial cells (NRK-52E) were detected using formazan assay and flow cytometry analysis, respectively. The expression levels of cleaved caspase-3, autophagy maker LC3 I/II, and AMPK/mTOR signaling pathway-related proteins were measured with Western blot analysis. The changes of morphology and RFP-LC3 fluorescence were observed under microscopy. RESULTS: Cisplatin (10-50 ?mol/L) dose-dependently induced cell damage and apoptosis in NRK-52E cells, whereas emodin (10 and 100 ?mol/L) significantly ameliorated cisplatin-induced cell damage, apoptosis and caspase-3 cleavage. Emodin dose-dependently increased LC3-II levels and induced RFP-LC3-containing punctate structures in NRK-52E cells. Furthermore, the protective effects of emodin were abolished by bafilomycin A1 (10 nmol/L), and mimicked by rapamycin (100 nmol/L). Moreover, emodin increased the phosphorylation of AMPK and suppressed the phosphorylation of mTOR. The AMPK inhibitor compound C (10 ?mol/L) not only abolished emodin-induced autophagy activation, but also emodin-induced anti-apoptotic effects. CONCLUSION: Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro through modulating the AMPK/mTOR signaling pathways and activating autophagy. Emodin may have therapeutic potential for the prevention of cisplatin-induced nephrotoxicity. Twit Text FOAVip Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy ????Acta Pharmacol Sin http://www.kidney.de/pget.php?&tw=1&pmid=26775661 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26775661 #FOAvip English Wikipedia <ref>{{Cite pmid|26775661 }}</ref> Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy #MMPMID26775661 Liu H ; Gu LB ; Tu Y ; Hu H ; Huang YR ; Sun W Acta Pharmacol Sin 2016[Feb]; 37 (2 ): 235-45 PMID26775661 show ga AIM: A previous report shows that emodin extracted from the Chinese herbs rhubarb and giant knotweed rhizome can ameliorate the anticancer drug cisplatin-induced injury of HEK293 cells. In this study, we investigated whether and how emodin could protect renal tubular epithelial cells against cisplatin-induced nephrotoxicity in vitro. METHODS: The viability and apoptosis of normal rat renal tubular epithelial cells (NRK-52E) were detected using formazan assay and flow cytometry analysis, respectively. The expression levels of cleaved caspase-3, autophagy maker LC3 I/II, and AMPK/mTOR signaling pathway-related proteins were measured with Western blot analysis. The changes of morphology and RFP-LC3 fluorescence were observed under microscopy. RESULTS: Cisplatin (10-50 ?mol/L) dose-dependently induced cell damage and apoptosis in NRK-52E cells, whereas emodin (10 and 100 ?mol/L) significantly ameliorated cisplatin-induced cell damage, apoptosis and caspase-3 cleavage. Emodin dose-dependently increased LC3-II levels and induced RFP-LC3-containing punctate structures in NRK-52E cells. Furthermore, the protective effects of emodin were abolished by bafilomycin A1 (10 nmol/L), and mimicked by rapamycin (100 nmol/L). Moreover, emodin increased the phosphorylation of AMPK and suppressed the phosphorylation of mTOR. The AMPK inhibitor compound C (10 ?mol/L) not only abolished emodin-induced autophagy activation, but also emodin-induced anti-apoptotic effects. CONCLUSION: Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro through modulating the AMPK/mTOR signaling pathways and activating autophagy. Emodin may have therapeutic potential for the prevention of cisplatin-induced nephrotoxicity. |AMP-Activated Protein Kinases/metabolism [MESH] |Animals [MESH] |Antineoplastic Agents/*adverse effects [MESH] |Apoptosis/drug effects [MESH] |Autophagy/*drug effects [MESH] |Caspase 3/metabolism [MESH] |Cell Line [MESH] |Cisplatin/*adverse effects [MESH] |Emodin/*pharmacology [MESH] |Humans [MESH] |Kidney Tubules/cytology/*drug effects/metabolism/pathology [MESH] |Protective Agents/*pharmacology [MESH] |Protein Kinase Inhibitors/*pharmacology [MESH] |Rats [MESH] |Signal Transduction/drug effects [MESH]Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular ce(epmc).pdf DeepDyve Pubget Overpricing