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10.1016/j.cmet.2015.11.014 http://scihub22266oqcxt.onion/10.1016/j.cmet.2015.11.014 C4752821!4752821!26712463     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Metab 2016 ; 23 (2): 379-85 Nephropedia Template TP {{tp|p=26712463|t=2016. Assessing the Mitochondrial Membrane Potential in Cells and In Vivo using Targeted Click Chemistry and Mass Spectrometry |pdf=|usr=}}{{26712463}} gab.com Text Assessing the Mitochondrial Membrane Potential in Cells and In Vivo using Targeted Click Chemistry and Mass Spectrometry JO: Cell Metab http://www.kidney.de/pget.php?&tw=1&pmid=26712463 #FOAvip The mitochondrial membrane potential (??m) is a major determinant and indicator of cell fate, but it is not possible to assess small changes in ??m within cells or in vivo. To overcome this, we developed an approach that utilizes two mitochondria-targeted probes each containing a triphenylphosphonium (TPP) lipophilic cation that drives their accumulation in response to ??m and the plasma membrane potential (??p). One probe contains an azido moiety and the other a cyclooctyne, which react together in a concentration-dependent manner by ?click? chemistry to form MitoClick. As the mitochondrial accumulation of both probes depends exponentially on ??m and ??p, the rate of MitoClick formation is exquisitely sensitive to small changes in these potentials. MitoClick accumulation can then be quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This approach enables assessment of subtle changes in membrane potentials within cells and in the mouse heart in vivo. Twit Text FOAVip Assessing the Mitochondrial Membrane Potential in Cells and In Vivo using Targeted Click Chemistry and Mass Spectrometry ????Cell Metab http://www.kidney.de/pget.php?&tw=1&pmid=26712463 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26712463 #FOAvip English Wikipedia <ref>{{Cite pmid|26712463}}</ref> Assessing the Mitochondrial Membrane Potential in Cells and In Vivo using Targeted Click Chemistry and Mass Spectrometry #MMPMID26712463 Logan A; Pell V; Shaffer K; Evans C; Stanley N; Robb E; Prime T; Chouchani E; Cochemé H; Fearnley I; Vidoni S; James A; Porteous C; Partridge L; Krieg T; Smith R; Murphy M Cell Metab 2016[Feb]; 23 (2): 379-85 PMID26712463show ga The mitochondrial membrane potential (??m) is a major determinant and indicator of cell fate, but it is not possible to assess small changes in ??m within cells or in vivo. To overcome this, we developed an approach that utilizes two mitochondria-targeted probes each containing a triphenylphosphonium (TPP) lipophilic cation that drives their accumulation in response to ??m and the plasma membrane potential (??p). One probe contains an azido moiety and the other a cyclooctyne, which react together in a concentration-dependent manner by ?click? chemistry to form MitoClick. As the mitochondrial accumulation of both probes depends exponentially on ??m and ??p, the rate of MitoClick formation is exquisitely sensitive to small changes in these potentials. MitoClick accumulation can then be quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This approach enables assessment of subtle changes in membrane potentials within cells and in the mouse heart in vivo. äAssessing the Mitochondrial Membrane Potential in Cells and In Vivo us(epmc).pdf DeepDyve Pubget Overpricing