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10.1016/j.stemcr.2016.01.006 http://scihub22266oqcxt.onion/10.1016/j.stemcr.2016.01.006 C4750161!4750161!26862703     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Reports 2016 ; 6 (2): 257-72 Nephropedia Template TP {{tp|p=26862703|t=2016. What Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast |pdf=|usr=}}{{26862703}} gab.com Text What Is Trophoblast A Combination of Criteria Define Human First-Trimester Trophoblast JO: Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=26862703 #FOAvip Controversy surrounds reports describing the derivation of human trophoblast cells from placentas and embryonic stem cells (ESC), partly due to the difficulty in identifying markers that define cells as belonging to the trophoblast lineage. We have selected criteria that are characteristic of primary first-trimester trophoblast: a set of protein markers, HLA class I profile, methylation of ELF5, and expression of microRNAs (miRNAs) from the chromosome 19 miRNA cluster (C19MC). We tested these criteria on cells previously reported to show some phenotypic characteristics of trophoblast: bone morphogenetic protein (BMP)-treated human ESC and 2102Ep, an embryonal carcinoma cell line. Both cell types only show some, but not all, of the four trophoblast criteria. Thus, BMP-treated human ESC have not fully differentiated to trophoblast. Our study identifies a robust panel, including both protein and non-protein-coding markers that, in combination, can be used to reliably define cells as characteristic of early trophoblast. Twit Text FOAVip What Is Trophoblast A Combination of Criteria Define Human First-Trimester Trophoblast ????Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=26862703 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26862703 #FOAvip English Wikipedia <ref>{{Cite pmid|26862703}}</ref> What Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast #MMPMID26862703 Lee C; Gardner L; Turco M; Zhao N; Murray M; Coleman N; Rossant J; Hemberger M; Moffett A Stem Cell Reports 2016[Feb]; 6 (2): 257-72 PMID26862703show ga Controversy surrounds reports describing the derivation of human trophoblast cells from placentas and embryonic stem cells (ESC), partly due to the difficulty in identifying markers that define cells as belonging to the trophoblast lineage. We have selected criteria that are characteristic of primary first-trimester trophoblast: a set of protein markers, HLA class I profile, methylation of ELF5, and expression of microRNAs (miRNAs) from the chromosome 19 miRNA cluster (C19MC). We tested these criteria on cells previously reported to show some phenotypic characteristics of trophoblast: bone morphogenetic protein (BMP)-treated human ESC and 2102Ep, an embryonal carcinoma cell line. Both cell types only show some, but not all, of the four trophoblast criteria. Thus, BMP-treated human ESC have not fully differentiated to trophoblast. Our study identifies a robust panel, including both protein and non-protein-coding markers that, in combination, can be used to reliably define cells as characteristic of early trophoblast. äWhat Is Trophoblast? A Combination of Criteria Define Human First-Trim(epmc).pdf DeepDyve Pubget Overpricing