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http://scihub22266oqcxt.onion/ C4747400!4747400!26517677     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Oncotarget 2015 ; 6 (38): 41204-15 Nephropedia Template TP {{tp|p=26517677|t=2015. Morphometric analysis of immunoselection against hyperploid cancer cells |pdf=|usr=}}{{26517677}} gab.com Text Morphometric analysis of immunoselection against hyperploid cancer cells JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=26517677 #FOAvip An at least transient increase of ploidy, usually by whole genome duplication, is a frequent event in oncogenesis, explaining the cytogenetic features of at least 40% of solid cancers. Here, we show that fibrosarcomas induced by the carcinogen methylcholanthrene (MCA) are distinct with respect to their ploidy status when they arise in immunocompetent wild type versus severely immunodeficient Rag2?/??c?/? mice. MCA-induced fibrosarcomas are particularly hyperploid if they develop in an immunodeficient setting, correlating with higher DNA content, increased nuclear surface, as well as hyperphosphorylation of eukaryotic initiation factor 2` (eIF2`), a biomarker indicating endoplasmic reticulum (ER) stress. Upon transfer of such cells into wild type mice, such hyperploid, ER-stressed cells (that originated in Rag2?/??c?/? mice) fail to proliferate and actually induce a protective anticancer immune response. In contrast, such cells do form tumors in Rag2?/??c?/? recipients (which lack T, B and NK cells) as well as in Rag2?/? recipients (which only lack T and B lymphocytes) and conserve their hyperploidy as well as eIF2` hyperphosphorylation. To measure these parameters, we developed a morphometric analysis tool that is applicable to immunohistochemistry of formaldehyde-fixed, paraffin-embedded tissues. This software automatically identifies and quantifies the surface of nuclei and determines the intensity of eIF2` phosphorylation within a perinuclear region of interest. Comparative analyses performed on cultured cells and tissue sections validated the accuracy of this method, which can be used to investigate ploidy and ER stress in cancers in situ. Twit Text FOAVip Morphometric analysis of immunoselection against hyperploid cancer cells ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=26517677 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26517677 #FOAvip English Wikipedia <ref>{{Cite pmid|26517677}}</ref> Morphometric analysis of immunoselection against hyperploid cancer cells #MMPMID26517677 Bloy N; Sauvat A; Chaba K; Buqué A; Humeau J; Bravo-San Pedro JM; Bui J; Kepp O; Kroemer G; Senovilla L Oncotarget 2015[Dec]; 6 (38): 41204-15 PMID26517677show ga An at least transient increase of ploidy, usually by whole genome duplication, is a frequent event in oncogenesis, explaining the cytogenetic features of at least 40% of solid cancers. Here, we show that fibrosarcomas induced by the carcinogen methylcholanthrene (MCA) are distinct with respect to their ploidy status when they arise in immunocompetent wild type versus severely immunodeficient Rag2?/??c?/? mice. MCA-induced fibrosarcomas are particularly hyperploid if they develop in an immunodeficient setting, correlating with higher DNA content, increased nuclear surface, as well as hyperphosphorylation of eukaryotic initiation factor 2` (eIF2`), a biomarker indicating endoplasmic reticulum (ER) stress. Upon transfer of such cells into wild type mice, such hyperploid, ER-stressed cells (that originated in Rag2?/??c?/? mice) fail to proliferate and actually induce a protective anticancer immune response. In contrast, such cells do form tumors in Rag2?/??c?/? recipients (which lack T, B and NK cells) as well as in Rag2?/? recipients (which only lack T and B lymphocytes) and conserve their hyperploidy as well as eIF2` hyperphosphorylation. To measure these parameters, we developed a morphometric analysis tool that is applicable to immunohistochemistry of formaldehyde-fixed, paraffin-embedded tissues. This software automatically identifies and quantifies the surface of nuclei and determines the intensity of eIF2` phosphorylation within a perinuclear region of interest. Comparative analyses performed on cultured cells and tissue sections validated the accuracy of this method, which can be used to investigate ploidy and ER stress in cancers in situ. äMorphometric analysis of immunoselection against hyperploid cancer cel(epmc).pdf DeepDyve Pubget Overpricing