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10.1038/ncomms10372 http://scihub22266oqcxt.onion/10.1038/ncomms10372 C4740111!4740111!26822409     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26822409|t=2016. Live-cell protein labelling with nanometre precision by cell squeezing |pdf=|usr=}}{{26822409}} gab.com Text Live-cell protein labelling with nanometre precision by cell squeezing JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26822409 #FOAvip Live-cell labelling techniques to visualize proteins with minimal disturbance are important; however, the currently available methods are limited in their labelling efficiency, specificity and cell permeability. We describe high-throughput protein labelling facilitated by minimalistic probes delivered to mammalian cells by microfluidic cell squeezing. High-affinity and target-specific tracing of proteins in various subcellular compartments is demonstrated, culminating in photoinduced labelling within live cells. Both the fine-tuned delivery of subnanomolar concentrations and the minimal size of the probe allow for live-cell super-resolution imaging with very low background and nanometre precision. This method is fast in probe delivery (?1,000,000 cells per second), versatile across cell types and can be readily transferred to a multitude of proteins. Moreover, the technique succeeds in combination with well-established methods to gain multiplexed labelling and has demonstrated potential to precisely trace target proteins, in live mammalian cells, by super-resolution microscopy. Twit Text FOAVip Live-cell protein labelling with nanometre precision by cell squeezing ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26822409 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26822409 #FOAvip English Wikipedia <ref>{{Cite pmid|26822409}}</ref> Live-cell protein labelling with nanometre precision by cell squeezing #MMPMID26822409 Kollmannsperger A; Sharei A; Raulf A; Heilemann M; Langer R; Jensen KF; Wieneke R; Tampé R Nat Commun 2016[]; 7 (ä): ä PMID26822409show ga Live-cell labelling techniques to visualize proteins with minimal disturbance are important; however, the currently available methods are limited in their labelling efficiency, specificity and cell permeability. We describe high-throughput protein labelling facilitated by minimalistic probes delivered to mammalian cells by microfluidic cell squeezing. High-affinity and target-specific tracing of proteins in various subcellular compartments is demonstrated, culminating in photoinduced labelling within live cells. Both the fine-tuned delivery of subnanomolar concentrations and the minimal size of the probe allow for live-cell super-resolution imaging with very low background and nanometre precision. This method is fast in probe delivery (?1,000,000 cells per second), versatile across cell types and can be readily transferred to a multitude of proteins. Moreover, the technique succeeds in combination with well-established methods to gain multiplexed labelling and has demonstrated potential to precisely trace target proteins, in live mammalian cells, by super-resolution microscopy. äLive-cell protein labelling with nanometre precision by cell squeezing(epmc).pdf DeepDyve Pubget Overpricing