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10.1038/ncomms10548 http://scihub22266oqcxt.onion/10.1038/ncomms10548 C4738357!4738357!26817820     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26817820|t=2016. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency |pdf=|usr=}}{{26817820}} gab.com Text RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26817820 #FOAvip Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are becoming major tools for genome editing. Importantly, knock-in in several non-rodent species has been finally achieved thanks to these customizable nucleases; yet the rates remain to be further improved. We hypothesize that inhibiting non-homologous end joining (NHEJ) or enhancing homology-directed repair (HDR) will improve the nuclease-mediated knock-in efficiency. Here we show that the in vitro application of an HDR enhancer, RS-1, increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects. We then apply RS-1 for animal production and have achieved multifold improvement on the knock-in rates as well. Our work presents tools to nuclease-mediated knock-in animal production, and sheds light on improving gene-targeting efficiencies on pluripotent stem cells. Twit Text FOAVip RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26817820 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26817820 #FOAvip English Wikipedia <ref>{{Cite pmid|26817820}}</ref> RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency #MMPMID26817820 Song J; Yang D; Xu J; Zhu T; Chen YE; Zhang J Nat Commun 2016[]; 7 (ä): ä PMID26817820show ga Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are becoming major tools for genome editing. Importantly, knock-in in several non-rodent species has been finally achieved thanks to these customizable nucleases; yet the rates remain to be further improved. We hypothesize that inhibiting non-homologous end joining (NHEJ) or enhancing homology-directed repair (HDR) will improve the nuclease-mediated knock-in efficiency. Here we show that the in vitro application of an HDR enhancer, RS-1, increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects. We then apply RS-1 for animal production and have achieved multifold improvement on the knock-in rates as well. Our work presents tools to nuclease-mediated knock-in animal production, and sheds light on improving gene-targeting efficiencies on pluripotent stem cells. äRS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency (epmc).pdf DeepDyve Pubget Overpricing