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10.1038/nbt.3290 http://scihub22266oqcxt.onion/10.1038/nbt.3290 C4729442!4729442!26121415     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2015 ; 33 (9): 985-9 Nephropedia Template TP {{tp|p=26121415|t=2015. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells |pdf=|usr=}}{{26121415}} gab.com Text Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=26121415 #FOAvip CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34+ hematopoietic stem and progenitor cells. Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery. This approach is a simple and effective way to streamline the development of genome editing with the potential to accelerate a wide array of biotechnological and therapeutic applications of the CRISPR-Cas technology. Twit Text FOAVip Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=26121415 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26121415 #FOAvip English Wikipedia <ref>{{Cite pmid|26121415}}</ref> Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells #MMPMID26121415 Hendel A; Bak RO; Clark JT; Kennedy AB; Ryan DE; Roy S; Steinfeld I; Lunstad BD; Kaiser RJ; Wilkens AB; Bacchetta R; Tsalenko A; Dellinger D; Bruhn L; Porteus MH Nat Biotechnol 2015[Sep]; 33 (9): 985-9 PMID26121415show ga CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34+ hematopoietic stem and progenitor cells. Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery. This approach is a simple and effective way to streamline the development of genome editing with the potential to accelerate a wide array of biotechnological and therapeutic applications of the CRISPR-Cas technology. äChemically modified guide RNAs enhance CRISPR-Cas genome editing in hu(epmc).pdf DeepDyve Pubget Overpricing