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10.1111/bph.13388 http://scihub22266oqcxt.onion/10.1111/bph.13388 C4728428!4728428!26565566     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Br+J+Pharmacol 2016 ; 173 (3): 575-87 Nephropedia Template TP {{tp|p=26565566|t=2016. APT070 (mirococept), a membrane?localizing C3 convertase inhibitor, attenuates early human islet allograft damage in vitro and in vivo in a humanized mouse model |pdf=|usr=}}{{26565566}} gab.com Text APT070 (mirococept), a membrane localizing C3 convertase inhibitor, attenuates early human islet allograft damage in vitro and in vivo in a humanized mouse model JO: Br J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=26565566 #FOAvip Background and Purpose: A major obstacle to islet cell transplantation is the early loss of transplanted islets resulting from the instant blood?mediated inflammation reaction (IBMIR). The activation of complement pathways plays a central role in IBMIR. The aim of this study was to test the inhibitory effect of ?painting? human islets with APT070, a membrane?localizing C3 convertase inhibitor, on inflammation evoked by exposure to human serum in vitro and by transplantation in vivo in a humanized diabetic mouse model. Experimental Approach: In vitro, human islets pre?incubated with APT070 were exposed to allogeneic whole blood. In vivo, similarly treated islets were transplanted underneath the kidney capsule of streptozotocin?induced diabetic NOD?SCID IL2r??/? mice that had been reconstituted with human CD34+ stem cells. Complement activation and islet hormone content were assayed using enzyme?linked immunosorbent assays. Supernatants and sera were assayed for cytokines using cytometric beads array. Morphology of the islets incubated with human serum in vitro and in graft?bearing kidney were evaluated using immunofluorescence staining. Key Results: Pre?incubation with APT070 decreased C?peptide release and iC3b production in vitro, with diminished deposition of C4d and C5b?9 in islets embedded in blood clots. In vivo, the APT070?treated islets maintained intact structure and showed less infiltration of inflammatory cells than untreated islets. The pretreatments also significantly reduced pro?inflammatory cytokines in supernatants and sera. Conclusions and Implications: Pre?treatment of islets with APT070 could reduce intra?islet inflammation with accompanying preservation of insulin secretion by beta cells. APT070 could be as a potential therapeutic tool in islet transplantation. Twit Text FOAVip APT070 (mirococept), a membrane localizing C3 convertase inhibitor, attenuates early human islet allograft damage in vitro and in vivo in a humanized mouse model ????Br J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=26565566 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26565566 #FOAvip English Wikipedia <ref>{{Cite pmid|26565566}}</ref> APT070 (mirococept), a membrane?localizing C3 convertase inhibitor, attenuates early human islet allograft damage in vitro and in vivo in a humanized mouse model #MMPMID26565566 Xiao F; Ma L; Zhao M; Smith RA; Huang G; Jones PM; Persaud S; Pingitore A; Dorling A; Lechler R; Lombardi G Br J Pharmacol 2016[Feb]; 173 (3): 575-87 PMID26565566show ga Background and Purpose: A major obstacle to islet cell transplantation is the early loss of transplanted islets resulting from the instant blood?mediated inflammation reaction (IBMIR). The activation of complement pathways plays a central role in IBMIR. The aim of this study was to test the inhibitory effect of ?painting? human islets with APT070, a membrane?localizing C3 convertase inhibitor, on inflammation evoked by exposure to human serum in vitro and by transplantation in vivo in a humanized diabetic mouse model. Experimental Approach: In vitro, human islets pre?incubated with APT070 were exposed to allogeneic whole blood. In vivo, similarly treated islets were transplanted underneath the kidney capsule of streptozotocin?induced diabetic NOD?SCID IL2r??/? mice that had been reconstituted with human CD34+ stem cells. Complement activation and islet hormone content were assayed using enzyme?linked immunosorbent assays. Supernatants and sera were assayed for cytokines using cytometric beads array. Morphology of the islets incubated with human serum in vitro and in graft?bearing kidney were evaluated using immunofluorescence staining. Key Results: Pre?incubation with APT070 decreased C?peptide release and iC3b production in vitro, with diminished deposition of C4d and C5b?9 in islets embedded in blood clots. In vivo, the APT070?treated islets maintained intact structure and showed less infiltration of inflammatory cells than untreated islets. The pretreatments also significantly reduced pro?inflammatory cytokines in supernatants and sera. Conclusions and Implications: Pre?treatment of islets with APT070 could reduce intra?islet inflammation with accompanying preservation of insulin secretion by beta cells. APT070 could be as a potential therapeutic tool in islet transplantation. äAPT070 (mirococept), a membrane?localizing C3 convertase inhibitor, at(epmc).pdf DeepDyve Pubget Overpricing