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Nanoemulsion Therapy for Burn Wounds Is Effective as a Topical Antimicrobial
Against Gram-Negative and Gram-Positive Bacteria
#MMPMID26182074
Dolgachev VA
; Ciotti SM
; Eisma R
; Gracon S
; Wilkinson JE
; Baker JR Jr
; Hemmila MR
J Burn Care Res
2016[Mar]; 37
(2
): e104-14
PMID26182074
show ga
The aim of this study is to investigate the antimicrobial efficacy of two
different nanoemulsion (NE) formulations against Gram-positive and Gram-negative
bacteria in an in vivo rodent scald burn model. Male Sprague-Dawley rats were
anesthetized and received a partial-thickness scald burn. Eight hours after burn
injury, the wound was inoculated with 1 × 10(8) colony-forming units of
Pseudomonas aeruginosa or Staphylococcus aureus. Treatment groups consisted of
two different NE formulations (NB-201 and NB-402), NE vehicle, or saline. Topical
application of the treatment was performed at 16 and 24 hours after burn injury.
Animals were killed 32 hours after burn injury, and skin samples were obtained
for quantitative wound culture and determination of dermal inflammation markers.
In a separate set of experiments, burn wound progression was measured
histologically after 72 hours of treatment. Both NE formulations (NB-201 and
NB-402) significantly reduced burn wound infections with either P. aeruginosa or
S. aureus and decreased median bacterial counts at least three logs when compared
with animals with saline applications (p < .0001). NB-201 and NB-402 also
decreased dermal neutrophil recruitment and sequestration into the wound as
measured by myeloperoxidase (MPO) assay and histopathology (p < .05). In
addition, there was a decrease in the proinflammatory dermal cytokines
(interleukin 1-beta [IL-1?], IL-6, and tumor necrosis factor alpha [TNF-?]) and
the neutrophil chemoattractants CXCL1 and CXCL2. Using histologic examination, it
was found that both NB-201 and NB-402 appeared to suppress burn wound progression
72 hours after injury. Topically applied NB-201 and NB-402 are effective in
decreasing Gram-positive and Gram-negative bacteria growth in burn wounds,
reducing inflammation, and abrogating burn wound progression.
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