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10.1002/anie.201505277 http://scihub22266oqcxt.onion/10.1002/anie.201505277 C4711269!4711269!26252716     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2015 ; 54 (40): 11706-10 Nephropedia Template TP {{tp|p=26252716|t=2015. Holliday junctions generate super-bright antibodies and antibody fragments in sortase-catalyzed reactions |pdf=|usr=}}{{26252716}} gab.com Text Holliday junctions generate super-bright antibodies and antibody fragments in sortase-catalyzed reactions JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip Site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching of multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide-modified, fluorescent single domain antibody fragment or an intact immunoglobulin, produced in a sortase-catalyzed reaction. We conclude that fluorescent Holliday junctions improve fluorescence yields for both single domain and full-sized antibodies, without deleterious effects on antigen binding. Twit Text FOAVip Holliday junctions generate super-bright antibodies and antibody fragments in sortase-catalyzed reactions ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip English Wikipedia <ref>{{Cite pmid|26252716}}</ref> Holliday junctions generate super-bright antibodies and antibody fragments in sortase-catalyzed reactions #MMPMID26252716 Li Z; Theile CS; Chen GY; Bilate AM; Duarte JN; Avalos AM; Fang T; Barberena R; Sato S; Ploegh HL Angew Chem Int Ed Engl 2015[Sep]; 54 (40): 11706-10 PMID26252716show ga Site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching of multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide-modified, fluorescent single domain antibody fragment or an intact immunoglobulin, produced in a sortase-catalyzed reaction. We conclude that fluorescent Holliday junctions improve fluorescence yields for both single domain and full-sized antibodies, without deleterious effects on antigen binding. äHolliday junctions generate super-bright antibodies and antibody fragm(epmc).pdf DeepDyve Pubget Overpricing