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10.1002/anie.201505277 http://scihub22266oqcxt.onion/10.1002/anie.201505277 C4711269!4711269 !26252716     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26252716 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Angew+Chem+Int+Ed+Engl 2015 ; 54 (40 ): 11706-10 Nephropedia Template TP {{tp|p=26252716 |t=2015. Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments |pdf=|usr=}}{{26252716 }} gab.com Text Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip The site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide-modified fluorescent single-domain antibody fragment or an intact immunoglobulin produced in a sortase-catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single-domain and full-sized antibodies without deleterious effects on antigen binding. Twit Text FOAVip Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26252716 #FOAvip English Wikipedia <ref>{{Cite pmid|26252716 }}</ref> Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments #MMPMID26252716 Li Z ; Theile CS ; Chen GY ; Bilate AM ; Duarte JN ; Avalos AM ; Fang T ; Barberena R ; Sato S ; Ploegh HL Angew Chem Int Ed Engl 2015[Sep]; 54 (40 ): 11706-10 PMID26252716 show ga The site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide-modified fluorescent single-domain antibody fragment or an intact immunoglobulin produced in a sortase-catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single-domain and full-sized antibodies without deleterious effects on antigen binding. |Alkynes/*chemistry [MESH] |Antibodies/*analysis/*chemistry [MESH] |Azides/*chemistry [MESH] |DNA, Cruciform/*chemistry [MESH]Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright (epmc).pdf DeepDyve Pubget Overpricing