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10.1117/1.NPh.2.4.041407 http://scihub22266oqcxt.onion/10.1117/1.NPh.2.4.041407 C4696521!4696521!26730396     free     free     free       Neurophotonics 2015 ; 2 (4): ä Nephropedia Template TP {{tp|p=26730396|t=2015. Ultramicroscopy: development and outlook |pdf=|usr=}}{{26730396}} gab.com Text Ultramicroscopy: development and outlook JO: Neurophotonics http://www.kidney.de/pget.php?&tw=1&pmid=26730396 #FOAvip We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, we designed a light sheet microscope and a chemical clearing to image complete mouse brains. Fluorescence of green fluorescent protein (GFP)-labeled neurons in mouse brains could be preserved with our 3DISCO clearing and high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used to image whole mouse embryos and flies. We improved the optical sectioning of our light sheet microscope by generating longer and thinner light sheets with aspheric optics. To obtain high-resolution images, we corrected available air microscope objectives for clearing solutions with high refractive index. We discuss how eventually super resolution could be realized in light sheet microscopy by applying stimulated emission depletion technology. Also the imaging of brain function by recording of mouse brains expressing cfos-GFP is discussed. Finally, we show the first 3-D recordings of human breast cancer with light sheet microscopy as application in medical diagnostics. Twit Text FOAVip Ultramicroscopy: development and outlook ????Neurophotonics http://www.kidney.de/pget.php?&tw=1&pmid=26730396 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26730396 #FOAvip English Wikipedia <ref>{{Cite pmid|26730396}}</ref> Ultramicroscopy: development and outlook #MMPMID26730396 Dodt HU; Saghafi S; Becker K; Jährling N; Hahn C; Pende M; Wanis M; Niendorf A Neurophotonics 2015[Oct]; 2 (4): ä PMID26730396show ga We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, we designed a light sheet microscope and a chemical clearing to image complete mouse brains. Fluorescence of green fluorescent protein (GFP)-labeled neurons in mouse brains could be preserved with our 3DISCO clearing and high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used to image whole mouse embryos and flies. We improved the optical sectioning of our light sheet microscope by generating longer and thinner light sheets with aspheric optics. To obtain high-resolution images, we corrected available air microscope objectives for clearing solutions with high refractive index. We discuss how eventually super resolution could be realized in light sheet microscopy by applying stimulated emission depletion technology. Also the imaging of brain function by recording of mouse brains expressing cfos-GFP is discussed. Finally, we show the first 3-D recordings of human breast cancer with light sheet microscopy as application in medical diagnostics. äUltramicroscopy: development and outlook (epmc).pdf DeepDyve Pubget Overpricing