Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1091/mbc.E15-05-0319 http://scihub22266oqcxt.onion/10.1091/mbc.E15-05-0319 C4694764!4694764 !26538022     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26538022 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Biol+Cell 2016 ; 27 (1 ): 75-89 Nephropedia Template TP {{tp|p=26538022 |t=2016. A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for insulin-dependent GLUT4 exocytosis |pdf=|usr=}}{{26538022 }} gab.com Text A complex of Rab13 with MICAL-L2 and -actinin-4 is essential for insulin-dependent GLUT4 exocytosis JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26538022 #FOAvip Insulin promotes glucose uptake into skeletal muscle through recruitment of glucose transporter 4 (GLUT4) to the plasma membrane. Rab GTPases are molecular switches mobilizing intracellular vesicles, and Rab13 is necessary for insulin-regulated GLUT4-vesicle exocytic translocation in muscle cells. We show that Rab13 engages the scaffold protein MICAL-L2 in this process. RNA interference-mediated knockdown of MICAL-L2 or truncated MICAL-L2 (MICAL-L2-CT) impaired insulin-stimulated GLUT4 translocation. Insulin increased Rab13 binding to MICAL-L2, assessed by pull down and colocalization under confocal fluorescence and structured illumination microscopies. Association was also visualized at the cell periphery using TIRF microscopy. Insulin further increased binding of MICAL-L2 to ?-actinin-4 (ACTN4), a protein involved in GLUT4 translocation. Rab13, MICAL-L2, and ACTN4 formed an insulin-dependent complex assessed by pull down and confocal fluorescence imaging. Of note, GLUT4 associated with the complex in response to insulin, requiring the ACTN4-binding domain in MICAL-L2. This was demonstrated by pull down with distinct fragments of MICAL-L2 and confocal and structured illumination microscopies. Finally, expression of MICAL-L2-CT abrogated the insulin-dependent colocalization of Rab13 with ACTN4 or Rab13 with GLUT4. Our findings suggest that MICAL-L2 is an effector of insulin-activated Rab13, which links to GLUT4 through ACTN4, localizing GLUT4 vesicles at the muscle cell periphery to enable their fusion with the membrane. Twit Text FOAVip A complex of Rab13 with MICAL-L2 and -actinin-4 is essential for insulin-dependent GLUT4 exocytosis ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26538022 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26538022 #FOAvip English Wikipedia <ref>{{Cite pmid|26538022 }}</ref> A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for insulin-dependent GLUT4 exocytosis #MMPMID26538022 Sun Y ; Jaldin-Fincati J ; Liu Z ; Bilan PJ ; Klip A Mol Biol Cell 2016[Jan]; 27 (1 ): 75-89 PMID26538022 show ga Insulin promotes glucose uptake into skeletal muscle through recruitment of glucose transporter 4 (GLUT4) to the plasma membrane. Rab GTPases are molecular switches mobilizing intracellular vesicles, and Rab13 is necessary for insulin-regulated GLUT4-vesicle exocytic translocation in muscle cells. We show that Rab13 engages the scaffold protein MICAL-L2 in this process. RNA interference-mediated knockdown of MICAL-L2 or truncated MICAL-L2 (MICAL-L2-CT) impaired insulin-stimulated GLUT4 translocation. Insulin increased Rab13 binding to MICAL-L2, assessed by pull down and colocalization under confocal fluorescence and structured illumination microscopies. Association was also visualized at the cell periphery using TIRF microscopy. Insulin further increased binding of MICAL-L2 to ?-actinin-4 (ACTN4), a protein involved in GLUT4 translocation. Rab13, MICAL-L2, and ACTN4 formed an insulin-dependent complex assessed by pull down and confocal fluorescence imaging. Of note, GLUT4 associated with the complex in response to insulin, requiring the ACTN4-binding domain in MICAL-L2. This was demonstrated by pull down with distinct fragments of MICAL-L2 and confocal and structured illumination microscopies. Finally, expression of MICAL-L2-CT abrogated the insulin-dependent colocalization of Rab13 with ACTN4 or Rab13 with GLUT4. Our findings suggest that MICAL-L2 is an effector of insulin-activated Rab13, which links to GLUT4 through ACTN4, localizing GLUT4 vesicles at the muscle cell periphery to enable their fusion with the membrane. |Actinin/*metabolism [MESH] |Animals [MESH] |Cell Line [MESH] |Cell Membrane/metabolism [MESH] |Exocytosis/physiology [MESH] |GTP Phosphohydrolases/*metabolism [MESH] |Glucose Transporter Type 4/*metabolism [MESH] |Humans [MESH] |Insulin/metabolism/pharmacology [MESH] |Microfilament Proteins/*metabolism [MESH] |Muscle Cells/metabolism [MESH] |Protein Binding [MESH] |Protein Transport [MESH] |Rats [MESH] |Signal Transduction [MESH] |Tight Junctions/metabolism [MESH]A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for in(epmc).pdf DeepDyve Pubget Overpricing