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10.1016/j.cell.2015.10.055 http://scihub22266oqcxt.onion/10.1016/j.cell.2015.10.055 C4690471!4690471!26548953     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2015 ; 163 (5): 1138-52 Nephropedia Template TP {{tp|p=26548953|t=2015. Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures |pdf=|usr=}}{{26548953}} gab.com Text Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=26548953 #FOAvip Diverse repertoires of antigen-receptor genes that result from combinatorial splicing of coding segments by V(D)J recombination are hallmarks of vertebrate immunity. The (RAG1-RAG2)2 recombinase (RAG) recognizes recombination signal sequences (RSSs) containing a heptamer, a spacer of 12 or 23 base pairs, and a nonamer (12-RSS or 23-RSS) and introduces precise breaks at RSS-coding segment junctions. RAG forms synaptic complexes only with one 12-RSS and one 23-RSS, a dogma known as the 12/23 rule that governs the recombination fidelity. We report cryo-electron microscopy structures of synaptic RAG complexes at up to 3.4 Å resolution, which reveal a closed conformation with base flipping and base-specific recognition of RSSs. Distortion at RSS-coding segment junctions and base flipping in coding segments uncover the two-metal-ion catalytic mechanism. Induced asymmetry involving tilting of the nonamer-binding domain dimer of RAG1 upon binding of HMGB1-bent 12-RSS or 23-RSS underlies the molecular mechanism for the 12/23 rule. Twit Text FOAVip Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=26548953 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26548953 #FOAvip English Wikipedia <ref>{{Cite pmid|26548953}}</ref> Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures #MMPMID26548953 Ru H; Chambers MG; Fu TM; Tong AB; Liao M; Wu H Cell 2015[Nov]; 163 (5): 1138-52 PMID26548953show ga Diverse repertoires of antigen-receptor genes that result from combinatorial splicing of coding segments by V(D)J recombination are hallmarks of vertebrate immunity. The (RAG1-RAG2)2 recombinase (RAG) recognizes recombination signal sequences (RSSs) containing a heptamer, a spacer of 12 or 23 base pairs, and a nonamer (12-RSS or 23-RSS) and introduces precise breaks at RSS-coding segment junctions. RAG forms synaptic complexes only with one 12-RSS and one 23-RSS, a dogma known as the 12/23 rule that governs the recombination fidelity. We report cryo-electron microscopy structures of synaptic RAG complexes at up to 3.4 Å resolution, which reveal a closed conformation with base flipping and base-specific recognition of RSSs. Distortion at RSS-coding segment junctions and base flipping in coding segments uncover the two-metal-ion catalytic mechanism. Induced asymmetry involving tilting of the nonamer-binding domain dimer of RAG1 upon binding of HMGB1-bent 12-RSS or 23-RSS underlies the molecular mechanism for the 12/23 rule. äMolecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Com(epmc).pdf DeepDyve Pubget Overpricing