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10.1038/nprot.2009.222 http://scihub22266oqcxt.onion/10.1038/nprot.2009.222 C4690457!4690457!20134419     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2010 ; 5 (2): 201-8 Nephropedia Template TP {{tp|p=20134419|t=2010. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice |pdf=|usr=}}{{20134419}} gab.com Text Thinned-skull cranial window technique for long-term imaging of the cortex in live mice JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=20134419 #FOAvip Imaging neurons, glia and vasculature in the living brain has become an important experimental tool for understanding how the brain works. Here we describe in detail a protocol for imaging cortical structures at high optical resolution through a thinned-skull cranial window in live mice using two-photon laser scanning microscopy (TPLSM). Surgery can be performed within 30?45 min and images can be acquired immediately thereafter. The procedure can be repeated multiple times allowing longitudinal imaging of the cortex over intervals ranging from days to years. Imaging through a thinned-skull cranial window avoids exposure of the meninges and the cortex, thus providing a minimally invasive approach for studying structural and functional changes of cells under normal and pathological conditions in the living brain. Twit Text FOAVip Thinned-skull cranial window technique for long-term imaging of the cortex in live mice ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=20134419 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20134419 #FOAvip English Wikipedia <ref>{{Cite pmid|20134419}}</ref> Thinned-skull cranial window technique for long-term imaging of the cortex in live mice #MMPMID20134419 Yang G; Pan F; Parkhurst CN; Grutzendler J; Gan WB Nat Protoc 2010[Feb]; 5 (2): 201-8 PMID20134419show ga Imaging neurons, glia and vasculature in the living brain has become an important experimental tool for understanding how the brain works. Here we describe in detail a protocol for imaging cortical structures at high optical resolution through a thinned-skull cranial window in live mice using two-photon laser scanning microscopy (TPLSM). Surgery can be performed within 30?45 min and images can be acquired immediately thereafter. The procedure can be repeated multiple times allowing longitudinal imaging of the cortex over intervals ranging from days to years. Imaging through a thinned-skull cranial window avoids exposure of the meninges and the cortex, thus providing a minimally invasive approach for studying structural and functional changes of cells under normal and pathological conditions in the living brain. äThinned-skull cranial window technique for long-term imaging of the co(epmc).pdf DeepDyve Pubget Overpricing