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10.1117/1.JBO.20.9.096016 http://scihub22266oqcxt.onion/10.1117/1.JBO.20.9.096016 C4688913!4688913!26405822     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biomed+Opt 2015 ; 20 (9): ä Nephropedia Template TP {{tp|p=26405822|t=2015. Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy |pdf=|usr=}}{{26405822}} gab.com Text Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy JO: J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=26405822 #FOAvip Fluorescence-based single molecule techniques to interrogate gene expression in tissues present a very low signal-to-noise ratio due to the strong autofluorescence and other background signals from tissue sections. This report presents a background-free method using second-harmonic generation (SHG) nanocrystals as probes to quantify the messenger RNA (mRNA) of human epidermal growth receptor 2 (Her2) at single molecule resolution in specific phenotypes at single-cell resolution directly in tissues. Coherent SHG emission from individual barium titanium oxide (BTO) nanoprobes was demonstrated, allowing for a stable signal beyond the autofluorescence window. Her2 surface marker and Her2 mRNA were specifically labeled with BTO probes, and Her2 mRNA was quantified at single copy sensitivity in Her2 expressing phenotypes directly in cancer tissues. Our approach provides the first proof of concept of a cross-platform strategy to probe tissues at single-cell resolution in situ. Twit Text FOAVip Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy ????J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=26405822 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26405822 #FOAvip English Wikipedia <ref>{{Cite pmid|26405822}}</ref> Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy #MMPMID26405822 Liu J; Damayanti NP; Cho IH; Polar Y; Badve S; Irudayaraj JMK J Biomed Opt 2015[Sep]; 20 (9): ä PMID26405822show ga Fluorescence-based single molecule techniques to interrogate gene expression in tissues present a very low signal-to-noise ratio due to the strong autofluorescence and other background signals from tissue sections. This report presents a background-free method using second-harmonic generation (SHG) nanocrystals as probes to quantify the messenger RNA (mRNA) of human epidermal growth receptor 2 (Her2) at single molecule resolution in specific phenotypes at single-cell resolution directly in tissues. Coherent SHG emission from individual barium titanium oxide (BTO) nanoprobes was demonstrated, allowing for a stable signal beyond the autofluorescence window. Her2 surface marker and Her2 mRNA were specifically labeled with BTO probes, and Her2 mRNA was quantified at single copy sensitivity in Her2 expressing phenotypes directly in cancer tissues. Our approach provides the first proof of concept of a cross-platform strategy to probe tissues at single-cell resolution in situ. äSingle-cell screening and quantification of transcripts in cancer tiss(epmc).pdf DeepDyve Pubget Overpricing