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10.1038/onc.2015.238

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suck abstract from ncbi


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pmid26096938      Oncogene 2016 ; 35 (13): 1725-35
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  • Translational regulation of Inhibin ?A by TGF? via the RNA-binding protein hnRNP E1 enhances the invasiveness of epithelial-to-mesenchymal transitioned cells #MMPMID26096938
  • Howley BV; Hussey GS; Link LA; Howe PH
  • Oncogene 2016[Mar]; 35 (13): 1725-35 PMID26096938show ga
  • The epithelial-to-mesenchymal transition (EMT) is a cellular process that functions during embryonic development and tissue regeneration, thought to be aberrantly activated in epithelial-derived cancer and play an important role in the process of metastasis. The TGF? signaling pathway is a key inducer of EMT and we have elucidated a post-transcriptional mechanism by which TGF? modulates expression of select transcripts via the RNA binding protein hnRNP E1 during EMT. One such transcript inhibin ?A is a member of the TGF? superfamily. Here, we show by polysome profiling that inhibin ?A is translationally regulated by TGF? via hnRNP E1. TGF? treatment or knockdown of hnRNP E1 relieves silencing of the inhibin ?A transcript, resulting in increased protein expression and secreted levels of the inhibin ?A homodimer, activin A. Our data indicates that the translational up-regulation of inhibin ?A enhances the migration and invasion of cells that have undergone an EMT and promotes cancer progression in vivo.
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