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10.1126/science.aaa5945 http://scihub22266oqcxt.onion/10.1126/science.aaa5945 C4687737!4687737!25908821     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25908821&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 500 Internal Server Error in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2015 ; 348 (6233): 442-4 Nephropedia Template TP {{tp|p=25908821|t=2015. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations |pdf=|usr=}}{{25908821}} gab.com Text The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype while individuals homozygous for two copies of the allele will display a mutant phenotype. Here, we develop a method that we refer to as the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome editing system for generating autocatalytic mutations to generate homozygous loss-of-function mutations. We demonstrate in Drosophila that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms. Twit Text FOAVip The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations ????Science http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25908821 #FOAvip English Wikipedia <ref>{{Cite pmid|25908821}}</ref> The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations #MMPMID25908821 Gantz VM; Bier E Science 2015[Apr]; 348 (6233): 442-4 PMID25908821show ga An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype while individuals homozygous for two copies of the allele will display a mutant phenotype. Here, we develop a method that we refer to as the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome editing system for generating autocatalytic mutations to generate homozygous loss-of-function mutations. We demonstrate in Drosophila that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms. äThe mutagenic chain reaction: a method for converting heterozygous to (epmc).pdf DeepDyve Pubget Overpricing