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10.1111/jcmm.12711 http://scihub22266oqcxt.onion/10.1111/jcmm.12711 C4687714!4687714!26471943     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Mol+Med 2015 ; 19 (12): 2741-50 Nephropedia Template TP {{tp|p=26471943|t=2015. In vitro morphology, viability and cytokine secretion of uterine telocyte?activated mouse peritoneal macrophages |pdf=|usr=}}{{26471943}} gab.com Text In vitro morphology, viability and cytokine secretion of uterine telocyte activated mouse peritoneal macrophages JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26471943 #FOAvip Telocytes (TCs), a distinct interstitial cell population, have been identified in the uterus, oviduct and placenta, with multiple proposed potential biological functions. Their unique structure allows them to form intercellular junctions with various immunocytes, both in normal and diseased tissues, suggesting a potential functional relationship with the local immune response. It has been hypothesized that through direct heterocellular junctions or indirect paracrine effects, TCs influence the activity of local immunocytes that are involved in the inflammatory process and in immune?mediated reproductive abnormalities. However, no reliable cytological evidence for this hypothesis is currently available. In this study, we cultured primary murine uterine TCs and collected TC conditioned media (TCM). Mouse peritoneal macrophages (pMACs) were co?cultured for 48 hrs with TCM or with DMEM/F12 or lipopolysaccharide (LPS) as negative and positive controls, respectively. Normal uterine TCs with a typical structure and a CD?34?positive/vimentin?positive/c?kit?negative immunophenotype were observed during culture. Morphologically, TCM?treated pMACs displayed an obvious activation/immunoresponse, in contrast to over?stimulation and cell death after LPS treatment and no sign of activation in the presence of DMEM/F12. Accordingly, a cell counting kit 8 (CCK?8) assay indicated significant activation of pMACs by TCM and LPS compared to DMEM/F12, thus supporting the marked morphological differences among these groups of cells. Furthermore, within a panel of macrophage?derived cytokines/enzymes, interleukin?6 (IL?6) and inducible nitric oxide synthase were significantly elevated in TCM?treated pMACs; tumour necrosis factor ?, IL1?R1, and IL?10 were slightly, but significantly, up?regulated; and no changes were observed for transforming growth factor??1, IL?1?, IL?23? and IL?18. Our results indicate that TCs are not simply innocent bystanders but are rather functional players in the activation of pMACs; they trigger and maintain the immune response, likely through indirect paracrine effects. Thus, we provide preliminary in vitro evidence of immunoregulatory and immunosurveillance roles for TCs. Twit Text FOAVip In vitro morphology, viability and cytokine secretion of uterine telocyte activated mouse peritoneal macrophages ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26471943 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26471943 #FOAvip English Wikipedia <ref>{{Cite pmid|26471943}}</ref> In vitro morphology, viability and cytokine secretion of uterine telocyte?activated mouse peritoneal macrophages #MMPMID26471943 Chi C; Jiang X; Su L; Shen Z; Yang X J Cell Mol Med 2015[Dec]; 19 (12): 2741-50 PMID26471943show ga Telocytes (TCs), a distinct interstitial cell population, have been identified in the uterus, oviduct and placenta, with multiple proposed potential biological functions. Their unique structure allows them to form intercellular junctions with various immunocytes, both in normal and diseased tissues, suggesting a potential functional relationship with the local immune response. It has been hypothesized that through direct heterocellular junctions or indirect paracrine effects, TCs influence the activity of local immunocytes that are involved in the inflammatory process and in immune?mediated reproductive abnormalities. However, no reliable cytological evidence for this hypothesis is currently available. In this study, we cultured primary murine uterine TCs and collected TC conditioned media (TCM). Mouse peritoneal macrophages (pMACs) were co?cultured for 48 hrs with TCM or with DMEM/F12 or lipopolysaccharide (LPS) as negative and positive controls, respectively. Normal uterine TCs with a typical structure and a CD?34?positive/vimentin?positive/c?kit?negative immunophenotype were observed during culture. Morphologically, TCM?treated pMACs displayed an obvious activation/immunoresponse, in contrast to over?stimulation and cell death after LPS treatment and no sign of activation in the presence of DMEM/F12. Accordingly, a cell counting kit 8 (CCK?8) assay indicated significant activation of pMACs by TCM and LPS compared to DMEM/F12, thus supporting the marked morphological differences among these groups of cells. Furthermore, within a panel of macrophage?derived cytokines/enzymes, interleukin?6 (IL?6) and inducible nitric oxide synthase were significantly elevated in TCM?treated pMACs; tumour necrosis factor ?, IL1?R1, and IL?10 were slightly, but significantly, up?regulated; and no changes were observed for transforming growth factor??1, IL?1?, IL?23? and IL?18. Our results indicate that TCs are not simply innocent bystanders but are rather functional players in the activation of pMACs; they trigger and maintain the immune response, likely through indirect paracrine effects. Thus, we provide preliminary in vitro evidence of immunoregulatory and immunosurveillance roles for TCs. äIn vitro morphology, viability and cytokine secretion of uterine teloc(epmc).pdf DeepDyve Pubget Overpricing